2021-05-26RELRCSBRCSBRCSB2021-02-242021-05-052021-05-052021-05-26David and Lucile Packard FoundationnaUnited StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)5R01GM115882-03United StatesMarseillevirus heterotrimeric (hexameric) nucleosomeValencia-Sanchez MIAbini-Agbomson SArmache K-JValencia-Sanchez MIAbini-Agbomson SWang MLee RVasilyev NZhang JDe Ioannes PLa Scola BTalbert PHenikoff SNudler EErives AArmache KJThe structure of a virus-encoded nucleosome.Nat.Struct.Mol.Biol.US28413417202133927388doi:10.1038/s41594-021-00585-71545-9985EMD-23529other EM volumeStructure of the Marseillevirus nucleosomeEMD-23530associated EM volumeMarseillevirus heterotrimeric (hexameric) nucleosome7lv9FULLOVERLAPMarseillevirus heterotrimeric (hexameric) nucleosomeMarseillevirus heterotrimeric (hexameric) nucleosome0123456virus-encoded
histone doublets
Marseillevirussynthetic constructEscherichia coliHistone doublet Delta-Gamma (Delta)Marseillevirus marseillevirus0.0104624159999999992Escherichia coliLEVOLADHVSVGETQIPKASTQHLLRKAGSLSAAGDTEVPIRGFVHMKLHKLVQKSLLAMQLAKRKTIMKSDVKKAAELMHLPV
FAIPTKDSGAKGSVFLSHistone doublet Delta-Gamma (Gamma)Marseillevirus marseillevirus0.01176762Escherichia coliLEVOCRQKGAGSAGTGSETNSQEVRSQMRSTCLIIPKERFRTMAKEISKKEGHDVHIAEAALDMLQVIVESCTVRLLEKALVIT
YSGKRTRVTSKDIETAFMLEHGPLLEHistone doublet Beta-Alpha (Beta)Marseillevirus marseillevirus0.0112280211Escherichia coliLEVOMATQKETTRKRDKSVNFRLGLRNMLAQIHPDISVQTEALSELSNIAVFLGKKISHGAVTLLPEGTKTIKSSAVLLAAGDL
YGKDLGRHAVGEMTKAVTRYGSAKHistone doublet Beta-Alpha (Alpha)Marseillevirus marseillevirus0.0176756251Escherichia coliLEVOESKEGSRSSKAKLQISVARSERLLREHGGCSRVSEGAAVALAAAIEYFMGEVLELAGNAARDSKKVRISVKHITLAIQND
AALFAVVGKGVFSGAGVSLISVPIPRKKARKTTEKEASSPKKKAAPKKKKAASKQKKSLSDKELAKLTKKELAKYEKEQG
MSPGYDNA (96-MER)synthetic construct0.029087571(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)
(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DG)(DA)(DT)(DT)(DC)(DT)(DC)(DC)
(DC)(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)
(DG)(DG)(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)
(DA)(DG)(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)DNADNA (96-MER)synthetic construct0.0295278261(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)
(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)
(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DG)(DA)
(DA)(DT)(DC)(DC)(DG)(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)
(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)DNAsingleParticleparticle3.37.520.0HepesHepes1.0EDTAEDTA2.0DTTDTTQuantifoil R1.2/1.3GOLD400CARBONHOLEYGLOW DISCHARGEETHANE100297FEI VITROBOT MARK IVFEI TITAN KRIOSOTHERBRIGHT FIELDFIELD EMISSION GUN3002.71.02.464000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K3 (6k x 4k)145032.565.013017414CTFFINDcryoSPARCAb initio in CryoSparc1C1FOURIER SPACE4.5FSC 0.143 CUT-OFFcryoSPARC128907OTHERcryoSPARC2.15Ab initio in CryoSparcPROJECTION MATCHINGcryoSPARC2.15RIGID BODY FITemd_23530_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
256
256256
0
00256256256279.04279.04279.0490.090.090.0XYZ-6.99997515.023239-0.000233123851.00441941.091.091.09Half map B Structure of the Marseillevirus Hexameremd_23530_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
256
256256
0
00256256256279.04279.04279.0490.090.090.0XYZ-7.114147714.477794-0.000411426181.00440191.091.091.09Half map A Structure of the Marseillevirus Hexamer