2021-05-12RELRCSBRCSBRCSB2021-03-052021-04-142021-04-142021-05-12National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM120553United StatesSARS-CoV-2 S/S2M11/S2X333 Global RefinementMcCallum MVeesler DSeattle Structural Genomics Center for Infectious Disease (SSGCID)McCallum MDe Marco ALempp FATortorici MAPinto DWalls ACBeltramello MChen ALiu ZZatta FZepeda Sdi Iulio JBowen JEMontiel-Ruiz MZhou JRosen LEBianchi SGuarino BFregni CSAbdelnabi RFoo SCRothlauf PWBloyet LMBenigni FCameroni ENeyts JRiva ASnell GTelenti AWhelan SPJVirgin HWCorti DPizzuto MSVeesler DN-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2.Cell1842332202133761326doi:10.1016/j.cell.2021.03.0281097-4172EMD-23579associated EM volumeSARS-CoV-2 S/S2M11/S2X333 Global Refinement7lxyFULLOVERLAPSARS-CoV-2 S hexapro bound to S2X333 FabSARS-CoV-2 S hexapro bound to S2X333 Fab012345Homo sapiensHomo sapiensSpike glycoproteinSevere acute respiratory syndrome coronavirus 20.1424274383Homo sapiensLEVOMFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFD
NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT
LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRV
QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC
NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFL
PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGS
NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI
SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGF
NFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAG
TITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQDVVNQNAQALN
TLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRV
DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNT
FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL
QELGKYEQGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSA
WSHPQFEKS2M11 Fab Light Chain variable regionHomo sapiens0.0112084583Homo sapiensLEVOIVMMQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEP
EDFAVYYCQQYGSSAWTFGQGTKVS2M11 Fab Heavy Chain variable regionHomo sapiens0.0136512199999999993Homo sapiensLEVOVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGWINPISSGTSYAQTFQGRVTMTSDTSITTAYM
ELSRLRSDDTAVYYCARAAPFYDFWSGYSYFDYWGQGTLVTVS2X333 Fab Heavy Chain variable regionHomo sapiens0.013391933Homo sapiensLEVOVQLVESGGGVVQPGRSLRLSCAASGFTFSNYGMHWVRQAPGKGLEWVAVIWYDGSNKFYADSVKGRFTISRDNSKNSLYL
QMNSLRAEDTAVYFCARAFPDSSSWSGFTIDYWGQGTLVTVS2X333 Fab Light Chain variable regionHomo sapiens0.0107506753Homo sapiensLEVOYELTQPPSVSTARITCGGNNIERKSVHWCQQKPGQAPALVVYDDSDRPSGIPERFSGSNSGNTATLTISRVEAGDEADYY
CQVWDSGSDQVIFGGGTKLT2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120845NAGwater1.8015e-05729HOHsingleParticleparticle8.020.0Tris-HCl150.0Sodium ChlorideNONEEthanefETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)COUNTING60.016ZGE2.2FSC 0.143 CUT-OFF261953MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_23579_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
600
600600
0
00600600600537.60004537.60004537.6000490.090.090.0XYZ-1.02825163.08364060.00066585160.0535056330.89600010.89600010.8960001Unsharpened mapemd_23579_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
600
600600
0
00600600600537.60004537.60004537.6000490.090.090.0XYZ-1.10542293.28136830.00166935820.129683290.89600010.89600010.8960001Half map Aemd_23579_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
600
600600
0
00600600600537.60004537.60004537.6000490.090.090.0XYZ-1.03072873.24734930.0017068980.129744680.89600010.89600010.8960001Half map B