2021-05-12RELRCSBRCSBRCSB2021-03-052021-04-142021-04-142021-05-12National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM120553United StatesSARS-CoV-2 S/S2M11/S2L28 Global RefinementMcCallum MVeesler DSeattle Structural Genomics Center for Infectious Disease (SSGCID)McCallum MDe Marco ALempp FATortorici MAPinto DWalls ACBeltramello MChen ALiu ZZatta FZepeda Sdi Iulio JBowen JEMontiel-Ruiz MZhou JRosen LEBianchi SGuarino BFregni CSAbdelnabi RFoo SCRothlauf PWBloyet LMBenigni FCameroni ENeyts JRiva ASnell GTelenti AWhelan SPJVirgin HWCorti DPizzuto MSVeesler DN-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2.Cell1842332202133761326doi:10.1016/j.cell.2021.03.0281097-4172EMD-23580associated EM volumeSARS-CoV-2 S/S2M11/S2L28 Global Refinement7lxzFULLOVERLAPSARS-CoV-2 S hexapro bound to S2L28 FabSARS-CoV-2 S hexapro bound to S2L28 Fab012345Homo sapiensHomo sapiensSpike glycoproteinSevere acute respiratory syndrome coronavirus 20.1424274383Homo sapiensLEVOMFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFD
NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT
LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRV
QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC
NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFL
PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGS
NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI
SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGF
NFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAG
TITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQDVVNQNAQALN
TLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRV
DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNT
FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL
QELGKYEQGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSA
WSHPQFEKS2M11 Fab Light Chain variable regionHomo sapiens0.0112084583Homo sapiensLEVOIVMMQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEP
EDFAVYYCQQYGSSAWTFGQGTKVS2M11 Fab Heavy Chain variable regionHomo sapiens0.0136512199999999993Homo sapiensLEVOVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGWINPISSGTSYAQTFQGRVTMTSDTSITTAYM
ELSRLRSDDTAVYYCARAAPFYDFWSGYSYFDYWGQGTLVTVS2L28 Fab Heavy Chain variable regionHomo sapiens0.0138492993Homo sapiensLEVOEVQLVESGGGLVNPGGSLRLSCAASGFTFSDYTIHWVRQAPGKGLEWVSSISSSSNYIYYADSVKGRFTISRDNAKNSLS
LQMNSLRAEDTAVYYCARDGNAYKWLLAENVRFDYWGQGTLVTVSSS2L28 Fab Light Chain variable regionHomo sapiens0.0111830543Homo sapiensLEVOVTQPASVSGSPGQSITISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYDVSDRPSGVSNRFSGSKSGNTASLTISGLQAE
DEADYYCSSYTSSSTPNWVFGGGTKLT2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120839NAGsingleParticleparticle8.020.0Tris-HCl150.0sodium chlorideETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)COUNTING60.016ZGE2.6FSC 0.143 CUT-OFF157770MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_23580_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400419.99997419.99997419.9999790.090.090.0XYZ-1.00170022.45087480.000827034240.0582708871.051.051.05Unsharpened mapemd_23580_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400419.99997419.99997419.9999790.090.090.0XYZ-1.28842942.69548340.00194838880.129006191.051.051.05Half map Bemd_23580_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400419.99997419.99997419.9999790.090.090.0XYZ-1.05054822.77106170.00194412990.128922631.051.051.05Half map A