2021-11-17
REL
RCSB
RCSB
RCSB
2021-03-15
2021-09-29
2021-09-29
2021-11-17
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
1R35GM133611
United States
National Science Foundation (NSF, United States)
1942049
United States
Cryo-EM Structure of Nucleosome containing mouse histone variant H2A.Z
Tan D
Lewis T
Lewis TS
Sokolova V
Jung H
Ng H
Tan D
Structural basis of chromatin regulation by histone variant H2A.Z.
Nucleic Acids Res.
UK
49
11379
11391
2021
34643712
doi:10.1093/nar/gkab907
1362-4962
0389
NARHAD
EMD-23626
associated EM volume
Cryo-EM Structure of Nucleosome containing mouse histone variant H2A.Z
7m1x
FULLOVERLAP
Nucleosome core particle containing variant H2A.Z and canonical core histones
Nucleosome core particle containing variant H2A.Z and canonical core histones
0
1
2
3
4
5
6
Histone H3
1
3
Xenopus laevis
Escherichia coli BL21(DE3)
Histone H4
1
4
Xenopus laevis
Escherichia coli BL21(DE3)
Histone variant H2A.Z
1
5
Mus musculus
Escherichia coli BL21(DE3)
Histone H2B
1
6
Xenopus laevis
Escherichia coli BL21(DE3)
DNA (136-MER)
1
1
unidentified
Escherichia coli DH5[alpha]
DNA (136-MER)
1
2
unidentified
Escherichia coli DH5[alpha]
DNA (136-MER)
unidentified
0.045604046999999995
1
(DA)(DC)(DA)(DG)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)
(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)
(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)
(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)
(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)
(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)
(DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)
(DT)(DC)(DT)(DC)(DC)(DA)(DG)
DNA
DNA (136-MER)
unidentified
0.045145753999999996
1
(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)
(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)
(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)
(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)
(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)
(DG)(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)
(DG)(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)
(DA)(DT)(DC)(DC)(DT)(DG)(DT)
DNA
Histone H3
Xenopus laevis
0.01550719
2
Escherichia coli BL21(DE3)
LEVO
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGEVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFK
TDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
Histone H4
Xenopus laevis
0.011394426
2
Escherichia coli BL21(DE3)
LEVO
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRK
TVTAMDVVYALKRQGRTLYGFGG
Histone H2A.Z
Mus musculus
0.013581796
2
Escherichia coli BL21(DE3)
LEVO
MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVK
RITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV
Histone H2B 1.1
Xenopus laevis
0.013979290999999998
2
Escherichia coli BL21(DE3)
LEVO
MPEPAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASR
LAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK
singleParticle
particle
1
8.0
2.5
NaCl
sodium chloride
10.0
Tris
2.0
EDTA
Ethylenediaminetetraacetic acid
5.0
BME
2-mercaptoethanol
Quantifoil R1.2/1.3
COPPER
400
CARBON
HOLEY
GLOW DISCHARGE
AIR
ETHANE
100
277.15
FEI VITROBOT MARK IV
blot for 4.5 seconds before plunging.
This sample was mono-disperse.
FEI TALOS ARCTICA
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
200
50.0
2.7
0.8
2.5
92000.0
123811.0
FEI TITAN KRIOS AUTOGRID HOLDER
NITROGEN
FEI FALCON III (4k x 4k)
COUNTING
3
1950
60.0
33.0
1
127778
CTFFIND
4.1.14
Generated in cryoSPARC
3
C1
BACK PROJECTION
3.7
FSC 0.143 CUT-OFF
RELION
3.1.0
42826
MAXIMUM LIKELIHOOD
RELION
3.1.0
MAXIMUM LIKELIHOOD
RELION
3.1.0
5
RELION
3.1.0
1F66
A
1F66
B
1F66
C
1F66
D
1F66
E
1F66
F
1F66
G
1F66
H
6FQ5
I
6FQ5
J
FLEXIBLE FIT
rmsd
REAL