2021-09-22RELRCSBRCSBRCSB2021-03-252021-09-222021-09-222021-09-22National Institutes of Health/National Institute on Aging (NIH/NIA)AG0543022United StatesCryo-EM structure of human islet amyloid polypeptide (hIAPP, or amylin) fibrils seeded by patient extracted fibrils, polymorph 4Cao QBoyer DRSawaya MREisenberg DSCao QBoyer DRSawaya MRAbskharon RSaelices LNguyen BALu JMurray KAKandeel FEisenberg DSCryo-EM structures of hIAPP fibrils seeded by patient-extracted fibrils reveal new polymorphs and conserved fibril coresNat.Struct.Mol.Biol.US287247302021doi:10.1038/s41594-021-00646-x1545-9985EMD-23689associated EM volumeEMD-23686other EM volumeEMD-23688other EM volumeEMD-23687other EM volume7m65FULLOVERLAPhIAPP fibrilhIAPP fibril01Synthetic hIAPP seeded by hIAPP fibrils extracted from a patient with Type II DiabetesHomo sapiensIslet amyloid polypeptideHomo sapiens0.00390731210LEVOKCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY(NH2)helicalfilament7.4Quantifoil R1.2/1.3COPPERGLOW DISCHARGEETHANE100298FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 (6k x 4k)SUPER-RESOLUTION345.014.81-2.94C14.1FSC 0.143 CUT-OFFRELION220365CTFFIND4.1.8NOT APPLICABLERELION2AB INITIO MODELREAL100.0