2021-08-25RELRCSBRCSBRCSB2021-05-132021-07-072021-07-072021-08-25National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL071818United StatesNational Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL122416United StatesNational Institutes of Health/National Cancer Institute (NIH/NCI)CA221289United StatesAmerican Heart Association19POST34450193United StatesRhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin and Fab1Chen QTesmer JJGChen QPlasencia MLi ZMukherjee SPatra DChen CLKlose TYao XQKossiakoff AAChang LAndrews PCTesmer JJGStructures of rhodopsin in complex with G-protein-coupled receptor kinase 1.NatureUK595600605202134262173doi:10.1038/s41586-021-03721-x1476-46870006NATUASEMD-23979associated EM volumeRhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin and Fab1EMD-23980other EM volumeEMD-23978other EM volumeEMD-23977other EM volume7mtaFULLOVERLAPRhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin stabilized by Fab1Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin stabilized by Fab101234Fab Heavy and Light Chains123Homo sapiensRhodopsin kinase, rhodopsin1Bos taurusRhodopsin kinase GRK1Bos taurus0.0615420121Spodoptera frugiperdaLEVOMDFGELETVVANSAFIAARGSFDASSGPASRDRKYLARLKLPPLSKCEALRESLDLGFEGMCLEQPIGKRLFQQFLRTHE
QHGPALQLWKDIEDYDTADDALRPQKAQALRAAYLEPQAQLFCSFLDAETVARARAGAGDGLFQPLLRAVLAHLGQAPFQ
EFLDSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGRGGFGEVFACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILA
KVHSRFIVSLAYAFETKTDLCLVMTIMNGGDIRYHIYNVDEDNPGFQEPRAIFYTAQIVSGLEHLHQRNIIYRDLKPENV
LLDDDGNVRISDLGLAVELKAGQTKTKGYAGTPGFMAPELLLGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKE
LKQRVLEQAVTYPDKFSPASKDFCEALLQKDPEKRLGFRDGSCDGLRTHPLFRDISWRQLEAGMLTPPFVPDSRTVYAKN
IQDVGAFEEVKGVAFEKADTEFFQEFASGTCPIPWQEEMIETGVFGDLNVWRPDGVDHHHHHH2.7.11.14Fab1 Heavy chainHomo sapiens0.0249997789999999961Escherichia coliLEVOEISEVQLVESGGGLVQPGGSLRLSCAASGFNLYSSSIHWVRQAPGKGLEWVASIYSYYGSTSYADSVKGRFTISADTSKN
TAYLQMNSLRAEDTAVYYCARYEGWWWANTYALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP
EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTFab1 Light chainHomo sapiens0.0237863959999999981Escherichia coliLEVOSDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQ
PEDFATYYCQQYYEWLSLFTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSG
NSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECRhodopsinBovine0.0390314571LEVOMNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLA
VADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFT
WVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQES
ATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTT
LCCGKNPLGDDEASTTVSKTETSQVAPASANGIVAMYCIN0.0003092781SGVRETINAL0.000284435999999999941RETsingleParticleparticle8.0ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 BIOQUANTUM (6k x 4k)54.014.1FSC 0.143 CUT-OFF310363OTHEROTHER