2021-11-17
REL
RCSB
RCSB
RCSB
2021-07-23
2021-09-22
2021-09-22
2021-11-17
Cryo-EM structure of human p97-R155H mutant bound to ATPgS.
Caffrey B
Zhu X
Berezuk A
Tuttle K
Chittori S
Subramaniam S
Caffrey B
Zhu X
Berezuk A
Tuttle K
Chittori S
Subramaniam S
AAA+ ATPase p97/VCP mutants and inhibitor binding disrupt inter-domain coupling and subsequent allosteric activation.
J.Biol.Chem.
US
297
101187
101187
2021
34520757
doi:10.1016/j.jbc.2021.101187
1083-351X
0071
JBCHA3
EMD-24519
associated EM volume
Cryo-EM structure of human p97-R155H mutant bound to ATPgS.
7rl7
FULLOVERLAP
Full-length Hexameric p97-R155H mutant.
Full-length Hexameric p97-R155H mutant.
0
1
Homo sapiens
Escherichia coli
BL21 DE3
0.540
Transitional endoplasmic reticulum ATPase
Homo sapiens
0.09121260199999999
6
Escherichia coli BL21(DE3)
LEVO
HHHHHHGTSENLYFQGASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRR
EAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRP
IRKGDIFLVHGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLR
HPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDE
LDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHT
KNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALR
ETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGP
ELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNR
PDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIES
EIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQ
GSGGGTGGSVYTEDNDDDLYG
3.6.4.6
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
0.0005232469999999999
12
AGS
MAGNESIUM ION
2.4305e-05
12
MG
singleParticle
twoDArray
2
8.0
150.0
NaCl
Sodium Chloride
50.0
C4H11NO3
Tris Hydrochloride
5.0
MgCl2
Magnesium Chloride
0.5
C14H28O6
Octyl Glucoside
1.0
C9H15O6P
TCEP
1.0
C10H12N5O12P3S
ATPgS
Protein Storage Buffer with ATPgS.
ETHANE
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
GATAN K3 (6k x 4k)
5265
50.0
1
1293922
3.0
FSC 0.143 CUT-OFF
284092
MAXIMUM LIKELIHOOD
MAXIMUM LIKELIHOOD