2021-11-24RELRCSBRCSBRCSB2021-07-292021-11-242021-11-242021-11-24National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01 GM118396National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32 GM007270Yeast CTP Synthase (Ura8) Bundle Bound to Substrates at Low pHHansen JMLynch EMFarrell DPDiMaio FQuispe JKollman JMHansen JMHorowitz ALynch EMFarrell DPQuispe JDiMaio FKollman JMCryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.ElifeUS10202134734801doi:10.7554/eLife.733682050-084XEMD-24581associated EM volumeYeast CTP Synthase (Ura8) Bundle Bound to Substrates at Low pH7rnrFULLOVERLAPCTP Synthase Bundle assembled with SubstratesCTP Synthase Bundle assembled with Substrates01Saccharomyces cerevisiaeEscherichia coliCTP synthaseSaccharomyces cerevisiae0.06243916799999999620Escherichia coliLEVOMKYVVVSGGVISGIGKGVLASSTGMLLKTLGLKVTSIKIDPYMNIDAGTMSPLEHGECFVLDDGGETDLDLGNYERYLGI
TLSRDHNITTGKIYSHVISRERRGDYLGKTVQIVPHLTNAIQDWIQRVSKIPVDDTGLEPDVCIIELGGTVGDIESAPFV
EALRQFQFEVGRENFALIHVSLVPVIHGEQKTKPTQAAIKDLRSLGLIPDMIACRCSEELNRSTIDKIAMFCHVGPEQVV
NVHDVNSTYHVPLLLLKQHMIDYLHSRLKLGEVPLTLEDKERGSQLLTNWENMTKNLDDSDDVVKIALVGKYTNLKDSYL
SVTKSLEHASMKCRRQLEILWVEASNLEPETQEVDKNKFHDSWNKLSSADGILVPGGFGTRGIEGMILAAKWARESGVPF
LGVCLGLQVAAIEFARNVIGRPNSSSTEFLDETLLAPEDQVVITMRLGLRPTIFQPNSEWSNIRKLYGEVNEVHERHRHR
YEINPKIVNDMESRGFIFVGKDETGQRCEIFELKGHPYYVGTQYHPEYTSKVLEPSRPFWGLVAAASGTLGEVIKDINL6.3.4.2ADENOSINE-5'-TRIPHOSPHATE0.00050718120ATPMAGNESIUM ION2.4305e-0540MGURIDINE 5'-TRIPHOSPHATE0.00048414120UTPsingleParticleparticle6.0ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)90.01C23.3OTHERFSCref0.5 (Phenix Density Modification)21220MAXIMUM LIKELIHOODMAXIMUM LIKELIHOOD