2022-02-09RELRCSBRCSBRCSB2021-12-212022-01-192022-01-192022-02-09National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM120553United StatesSARS-CoV-2 spike in complex with the S2K146 neutralizing antibody Fab fragment (three receptor-binding domains open)Park YJVeesler DPark YJDe Marco AStarr TNLiu ZPinto DWalls ACZatta FZepeda SKBowen JESprouse KRJoshi AGiurdanella MGuarino BNoack JAbdelnabi RFoo SCRosen LELempp FABenigni FSnell GNeyts JWhelan SPJVirgin HWBloom JDCorti DPizzuto MSVeesler DAntibody-mediated broad sarbecovirus neutralization through ACE2 molecular mimicry.ScienceUS375449454202234990214doi:10.1126/science.abm81431095-92030038SCIEASEMD-25785associated EM volumeSARS-CoV-2 spike in complex with the S2K146 neutralizing antibody Fab fragment (three receptor-binding domains open)SARS-CoV-2 spike in complex with the S2K146 neutralizing antibody Fab fragment (three receptor-binding domains open)0123Homo sapiensSARS-CoV-2 spike11Severe acute respiratory syndrome coronavirus 2Homo sapiensS2K146123Homo sapiensHomo sapiensSpike glycoproteinSevere acute respiratory syndrome coronavirus 2Homo sapiensLEVOMFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFD
NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT
LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRV
QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC
NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFL
PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGS
NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI
SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGF
NFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAG
TITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQDVVNQNAQALN
TLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRV
DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNT
FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL
QELGKYEQGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSA
WSHPQFEKS2K146 Fab heavy chainHomo sapiensHomo sapiensLEVOQVQLVESGGVVVQPGGSLRLSCAASGFTFHDHTMHWVRQAPGKGLEWVSLITWNGGTIHYSDSVKGRFTISRDNSKNSLYLQMNSLRTEDTALYYCAKDLGRGGWYLPSDAWGQGTLVTVSSS2K146 Fab light chainHomo sapiensHomo sapiensLEVOQSVLTQPPSASGTPGQRVTISCSGSSANIGSNTVNWYQHLPGTAPKLLIYSNNQRPSGVPDRFSGSKSGTSASLAISGLQSEDEADYYCAAWDDSLKGVFGGGTKLTVLsingleParticleparticle8.0ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3000.52.5GATAN K3 (6k x 4k)60.016VXX3.2FSC 0.143 CUT-OFFcryoSPARC212054PROJECTION MATCHINGPROJECTION MATCHINGemd_25785_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
512
512512
0
00512512512512.0512.0512.090.090.090.0XYZ-0.721731841.9352205-0.00202027520.033520771.01.01.0emd_25785_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
512
512512
0
00512512512512.0512.0512.090.090.090.0XYZ-0.868105952.2243499.324738e-050.071598811.01.01.0emd_25785_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)