RELPDBePDBePDBe2014-07-292014-09-032014-09-102014-09-17Conformational Snapshots of Inducible Nitric Oxide Synthase (iNOS)Campbell MGSmith BCPotter CSCarragher BMarletta MAheterogeneity, random conical tilt, nitric oxide synthase, calmodulin, heme, electron transfer, flavin,Campbell MGSmith BCPotter CSCarragher BMarletta MAMolecular architecture of mammalian nitric oxide synthases.PROC.NAT.ACAD.SCI.USA111E3614E3623201425125509doi:10.1073/pnas.1413763111Murine Inducible Nitric Oxide SynthaseMurine Inducible Nitric Oxide SynthaseSample is highly flexibleHomodimer10.26Inducible Nitric Oxide SynthaseMus musculusHouse mouseMacrophages0.261HomodimertrueEscherichia coliJM109pCWiNOSP29477GO:0006809GO:0010181GO:0050661GO:0005516GO:0050660GO:0020037GO:0005506GO:0004517IPR000333singleParticleparticle7.550 mM TEA pH 7.5, 150 mM NaCl, and 5 mM DTTNEGATIVE3 microliters of sample were applied to grid. The specimen was stained twice with 2% uranyl formate, then allowed to air-dry.Glow discharged C-flat grid with 2-micron-diameter holes overlaid by thin 1.5 nm continuous carbonNONEOTHERFEI TECNAI F20FLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN20021.02.562000.0114705.0SIDE ENTRY, EUCENTRIC2013-03-06TVIPS TEMCAM-F416 (4k x 4k)222637Each area is imaged twice: once at 55 degrees and again with no tilt.055See publication.Each ImageC1OTHER64.0OTHERAppion, SpiderSee publication.1931