2022-12-14RELRCSBRCSBRCSB2022-07-182022-12-072022-12-072022-12-14National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM14436101United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM136258United StatesCystic Fibrosis FoundationPACZKO21G0United StatesNational Science Foundation (NSF, United States)DMR-1719875United StatesQuorum-sensing receptor RhlR bound to PqsEPaczkowski JEFromme JCFeathers JRFeathers JRRichael EKSimanek KAFromme JCPaczkowski JEStructure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum-sensing gene regulation.StructureUK301626202236379213doi:10.1016/j.str.2022.10.0080969-21262005STRUE6EMD-27646associated EM volumeQuorum-sensing receptor RhlR bound to PqsE8dq1FULLOVERLAPTetrameric complex of RhlR:mBTL bound to PqsETetrameric complex of RhlR:mBTL bound to PqsE01234Pseudomonas aeruginosa.123RhlR proteinPseudomonas aeruginosa0.0276115962Escherichia coli 'BL21-Gold(DE3)pLysS AG'LEVOMRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAYGVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAV
DPAILNGLRSSEMVVWSDSLFDQSRMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIE
LLTQKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKKFDAPNKTLAAAYAAALGL
I2-aminobenzoylacetyl-CoA thioesterasePseudomonas aeruginosa0.0343502969999999952Escherichia coli 'BL21-Gold(DE3)pLysS AG'LEVOMLRLSAPGQLDDDLCLLGDVQVPVFLLRLGEASWALVEGGISRDAELVWADLCRWVADPSQVHYWLITHKHYDHCGLLPY
LCPRLPNVQVLASERTCQAWKSESAVRVVERLNRQLLRAEQRLPEACAWDALPVRAVADGEWLELGPRHRLQVIEAHGHS
DDHVVFYDVRRRRLFCGDALGEFDEAEGVWRPLVFDDMEAYLESLERLQRLPTLLQLIPGHGGLLRGRLAADGAESAYTE
CLRLCRRLLWRQSMGESLDELSEELHRAWGGQSVDFLPGELHLGSMRRMLEILSRQALPLDDNA (5'-D(*AP*CP*CP*TP*GP*CP*CP*AP*GP*AP*CP*TP*GP*CP*AP*CP*AP*G)-3')Pseudomonas aeruginosa0.00547055799999999951(DA)(DC)(DC)(DT)(DG)(DC)(DC)(DA)(DG)(DA)(DC)(DT)(DG)(DC)(DA)(DC)(DA)(DG)DNADNA (5'-D(*CP*TP*GP*TP*GP*CP*AP*GP*TP*CP*TP*GP*GP*CP*AP*GP*GP*T)-3')Pseudomonas aeruginosa0.00556358799999999951(DC)(DT)(DG)(DT)(DG)(DC)(DA)(DG)(DT)(DC)(DT)(DG)(DG)(DC)(DA)(DG)(DG)(DT)DNA4-(3-bromophenoxy)-N-[(3S)-2-oxothiolan-3-yl]butanamide0.000358250999999999972K5GsingleParticleparticle5.08.0ETHANEFEI TALOS ARCTICAFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN2000.81.5GATAN K3 BIOQUANTUM (6k x 4k)50.01EMD-276454.1FSC 0.143 CUT-OFF84842MAXIMUM LIKELIHOODRELIONMAXIMUM LIKELIHOODRELION4.0-betaemd_27646_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
208
208208
0
00208208208268.32268.32268.3290.090.090.0XYZ-0.0415125970.078869499.115322e-050.00164211161.29000011.29000011.2900001Sharpened post process mapemd_27646_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
208
208208
0
00208208208268.32268.32268.3290.090.090.0XYZ-0.0181649050.0387950065.9629005e-050.00191648891.29000011.29000011.2900001Density modified half map 1emd_27646_additional_3.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
208
208208
0
00208208208268.32268.32268.3290.090.090.0XYZ-0.0191987720.0388478756.2131534e-050.00192591841.29000011.29000011.2900001Density modified half map 2emd_27646_additional_4.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
208
208208
0
00208208208268.32268.32268.3290.090.090.0XYZ-0.0128273760.0341859056.0896135e-050.00108958051.29000011.29000011.2900001Refinement full mapemd_27646_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
208
208208
0
00208208208268.32268.32268.3290.090.090.0ZYX-1.75936252.47937139.070099e-060.0515016431.29000011.29000011.2900001Refinement half map 1emd_27646_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)