2020-12-16RELPDBjPDBjPDBj2020-09-142020-10-282020-10-282020-12-16Japan Society for the Promotion of Science (JSPS)18K06097JapanJapan Agency for Medical Research and Development (AMED)JP19am0101001 (support number 0053)JapanJapan Society for the Promotion of Science (JSPS)15K21608JapanJapan Science and TechnologyJPMJCR14M1JapanJapan Science and TechnologyJPMJCR13M7JapanJapan Society for the Promotion of Science (JSPS)25111004JapanJapan Agency for Medical Research and Development (AMED)JP19am0101079 (support number 0739)JapanJapan Society for the Promotion of Science (JSPS)19H05707JapanJapan Society for the Promotion of Science (JSPS)18H03989JapanCryo-EM structure of Schizosaccharomyces pombe Atg9 of trimerMatoba KTsutsumi AMatoba KKotani TTsutsumi ATsuji TMori TNoshiro DSugita YNomura NIwata SOhsumi YFujimoto TNakatogawa HKikkawa MNoda NNAtg9 is a lipid scramblase that mediates autophagosomal membrane expansion.Nat.Struct.Mol.Biol.US2711851193202033106658doi:10.1038/s41594-020-00518-w1545-9985EMD-30535other EM volumeEMD-30545associated EM volumeProtein detergent complex (Atg9-LMNG ) treated with GraFixProtein detergent complex (Atg9-LMNG ) treated with GraFix01Schizosaccharomyces pombeSaccharomyces cerevisiaeBJ926pRS426Atg9Schizosaccharomyces pombeSaccharomyces cerevisiaeLEVOMFYQPAQNKKQYDDLADIEAQNNVPNTQEVLEAWQESLDSDEDESSPLEESNGFTISEHDDFVKSVPRKNNPTDLLYSGK
LLDSDEPPSVHGNSSKVPSKHPSPSFPETTSLRNLQNGSKQKPALPNFNDPHFYNEDVTRSGHPNRSIYTQLPRNEFSNA
RVLWNRLSARDRVLWRWANVENLDSFLQQVYTYYTGKGLSCIIVHRLFQILTVSFVIGFTTFITSCIDWPAVTPHGSLAG
VTKSQCIAQMSPITYLVLWLFLSFLLALWIYYLTDIPRLWQMREFYIHALKIATADMPTVSWQRVLYRLLKLKNVNALTA
EDGRVVSLHNMKRLDAYAIANRIMRKDNYFIALINNGIINIELPLLHRRILTHTTEWNINWCIFNFVFDEQGQLRSAFRN
PNSRKRLSEELRRRFIVAGFLNCLFAPIVAIYLVIHNFFRYFNEYHKNPGALSTRRYTPLALWTFREYNELQHFFDERIN
DSYAAASHYVSQFPDFNMIRLFKYISFILGSFTAILVIITVFDPELMVTFEITKDRSVLFYLGLFGSLIAVSRSIIPDET
LVFAPEKALRRVITFTHYMPGWWSDNMHSKAVQQEFCSLYSYRIVNLLWEILGILLTPVLLFFTFPSCSQDIVDFFREHT
INVEGVGYVCSYAVFQDNPPYESVASLVQSRKISPLIQNKPELSRIYFYEQFNTEAPRRDLRGSLEVLFQsingleParticleparticle0.58.320.0HEPESN-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid150.0KClpotassium chloride0.002LMNGLauryl Maltose Neopentyl GlycolQuantifoil R1.2/1.3COPPER/RHODIUM300GLOW DISCHARGEETHANE100279FEI VITROBOT MARK IVFEI TALOS ARCTICAFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN2002.71.02.0130000.0NITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING8.050.014.7FSC 0.143 CUT-OFFRELION3.143113MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_30545_msk_1.mapemd_30545_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
140
140140
0
00140140140216.29999216.29999216.2999990.090.090.0XYZ-0.0268580.073494160.00136337320.005954941.5451.5451.545emd_30545_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)