2021-08-18RELPDBjPDBjPDBj2020-11-122021-08-042021-08-042021-08-18Japan Society for the Promotion of Science (JSPS)16H06294JapanDog beta3 adrenergic receptor bound to mirabegron in complex with a miniGs heterotrimerShihoya WYamashita KNureki ONagiri CKobayashi KTomita AKato MKobayashi KYamashita KNishizawa TInoue AShihoya WNureki OCryo-EM structure of the beta 3-adrenergic receptor reveals the molecular basis of subtype selectivity.Mol.CellUS8132053215.e5202134314699doi:10.1016/j.molcel.2021.06.0241097-27652168MOCEFLEMD-30678associated EM volumeDog beta3 adrenergic receptor bound to mirabegron in complex with a miniGs heterotrimer7dh5FULLOVERLAPDog beta3 adrenergic receptor bound to mirabegron in complex with a miniGs heterotrimerDog beta3 adrenergic receptor bound to mirabegron in complex with a miniGs heterotrimer012345Guanine nucleotide-binding protein G(s) subunit alpha isoforms short11Homo sapiensEscherichia coliGuanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-112Rattus norvegicusSpodoptera frugiperdaGuanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-213Bos taurusSpodoptera frugiperdananobody Nb3514unidentifiedEscherichia coliBeta-3 adrenergic receptor15Canis lupus familiarisSpodoptera frugiperdaGuanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortHomo sapiens0.0289647341Escherichia coliLEVOGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVD
KVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGK
SKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCRDIIQR
MHLRQYELLGuanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1Rattus norvegicus0.0387443711Spodoptera frugiperdaLEVOMHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRL
LVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCC
RFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLA
GHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGuanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2Bos taurus0.0075476851Spodoptera frugiperdaLEVOMASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFSnanobody Nb35unidentified0.0150157279999999991Escherichia coliLEVOMGQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNT
LYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSLHHHHHHBeta-3 adrenergic receptorCanis lupus familiaris0.0424412931Spodoptera frugiperdaLEVODYKDDDDAMGAPWPHGNGSVASWPAAPTPTPDAANTSGLPGAPWAVALAGALLALEVLATVGGNLLVIVAIARTPRLQTM
TNVFVTSLATADLVVGLLVVPPGATLALTGRWPLGATGCELWTSVDVLCVTASIETLCALAVDRYLAVTNPLRYGALVTK
RRARAAVVLVWVVSAAVSFAPIMSKWWRVGADAEAQRCHSNPHCCAFASNIPYALLSSSVSFYLPLLVMLFVYARVFLVA
TRQLRLLRRELGRFPPAESPPAASRSRSPGPARRCASPAAVPSDRLRPARLLPLREHRALRTLGLIVGTFTLCWLPFFVA
NVMRALGGPSLVPSPALLALNWLGYANSAFNPLIYCRSPDFRSAFRRLLCRCRREEHRAAASPPGDPSENLYFQG2-(2-azanyl-1,3-thiazol-4-yl)-N-[4-[2-[[(2R)-2-oxidanyl-2-phenyl-ethyl]amino]ethyl]phenyl]ethanamide0.0003965061H6UsingleParticleparticle78.0ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 (6k x 4k)50.013.16FSC 0.143 CUT-OFF539775MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_30678_msk_1.mapemd_30678_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
154
154154
0
00154154154159.77501159.77501159.7750190.090.090.0XYZ-0.0299315380.067725025-0.000365974470.00355735631.03751.03751.0375emd_30678_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)