2021-11-03RELPDBjPDBjPDBj2021-02-182021-05-052021-05-052021-11-03Japan Society for the Promotion of Science (JSPS)JP19K16088JapanJapan Society for the Promotion of Science (JSPS)JP18H05229JapanJapan Society for the Promotion of Science (JSPS)JP18H05534JapanJapan Society for the Promotion of Science (JSPS)JP18H03681JapanMinistry of Education, Culture, Sports, Science and Technology (Japan)JP16H06280JapanOpen type of human proteasome alpha 7 homo-tetradecamerSong CMurata KSong CSatoh TSekiguchi TKato KMurata KStructural Fluctuations of the Human Proteasome alpha 7 Homo-Tetradecamer Double Ring Imply the Proteasomal alpha-Ring Assembly Mechanism.Int J Mol SciCH22202133926037doi:10.3390/ijms220945191422-0067EMD-30990other EM volumeSymmetric typeEMD-30991other EM volumeDislocated typeEMD-30992associated EM volumeProteasome subunit alpha type-7Proteasome subunit alpha type-701Homo sapiensEscherichia colisingleParticleparticle1.07.5IceQuantifoil R1.2/1.3MOLYBDENUM200GLOW DISCHARGEETHANE95277JEOL 2200FSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN20040.04.21.03.040000.045065.0GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDERNITROGEN76.077.0In-column Omega Filter20DIRECT ELECTRON DE-20 (5k x 3k)512038406.45.040.01CTFFIND4C2BACK PROJECTION11.9FSC 0.143 CUT-OFFRELION3.11234MAXIMUM LIKELIHOODRELION3.1ANGULAR RECONSTITUTIONRELION3.1