REL
PDBe
PDBe
PDBe
2015-08-31
2015-09-23
2015-10-21
2015-10-21
A Single Alpha Helix Drives Extensive Remodeling of the Proteasome Lid and Completion of Regulatory Particle Assembly
Tomko Jr RJ
Taylor DW
Chen ZA
Wang HW
Rappsilber J
Hochstrasser M
proteasome, ubiquitin, proteolysis, electron microscopy, mass spectrometry
Tomko RJ
Taylor DW
Chen ZA
Wang HW
Rappsilber J
Hochstrasser M
A Single alpha Helix Drives Extensive Remodeling of the Proteasome Lid and Completion of Regulatory Particle Assembly.
CELL(CAMBRIDGE,MASS.)
163
432
444
2015
26451487
doi:10.1016/j.cell.2015.09.022
Recombinant LP2 (Lid Particle 2)
Recombinant LP2 (Lid Particle 2)
The sample was monodisperse.
1 Rpn3: 1 Rpn5: 1 Rpn6: 1 Rpn7: 1 Rpn8: 1 Rpn9: 1 Rpn11: 1 Sem1
8
0.343
26S proteasome regulatory subunit RPN3
Saccharomyces cerevisiae
Yeast
0.064
1
monomer
false
P40016
GO:0006511
GO:0042176
GO:0003674
GO:0000502
GO:0008541
IPR013586
IPR000717
26S proteasome regulatory subunit RPN5
Saccharomyces cerevisiae
Yeast
0.052
1
monomer
false
Q12250
GO:0006511
GO:0010388
GO:0003674
GO:0000502
GO:0008541
IPR000717
IPR011991
26S proteasome regulatory subunit RPN6
Saccharomyces cerevisiae
Yeast
0.050
1
monomer
false
Q12377
GO:0006511
GO:0005198
GO:0000502
GO:0008541
IPR000717
IPR013143
26S proteasome regulatory subunit RPN7
Saccharomyces cerevisiae
Yeast
0.049
1
monomer
false
Q06103
GO:0006511
GO:0005198
GO:0000502
GO:0008541
IPR019585
IPR000717
IPR013026
26S proteasome regulatory subunit RPN8
Saccharomyces cerevisiae
Yeast
0.038
1
monomer
false
Q08723
GO:0006511
GO:0003674
GO:0000502
GO:0008541
IPR000555
IPR024969
26S proteasome regulatory subunit RPN9
Saccharomyces cerevisiae
Yeast
0.046
1
monomer
false
Q04062
GO:0006511
GO:0005198
GO:0000502
GO:0008541
IPR000717
IPR011991
26S proteasome regulatory subunit RPN11
Saccharomyces cerevisiae
Yeast
0.034
1
monomer
false
P43588
GO:0016579
GO:0000266
GO:0004843
GO:0000502
GO:0008541
IPR000555
IPR024969
26S proteasome regulatory subunit Sem1
Saccharomyces cerevisiae
Yeast
0.010
1
monomer
false
O94742
GO:0000502
GO:0008541
GO:0043161
GO:0043248
IPR007834
singleParticle
particle
0.04
7.5
50 mM Tris, 150 mM NaCl, 5 mM MgCl2, and 10% glycerol
NEGATIVE
Sample was negatively stained with six consecutive droplets of 2% uranyl acetate.
400 mesh Cu grid with thin carbon, glow discharged with a sputter coater.
NONE
OTHER
FEI TECNAI 20
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
120
2.2
0.5
1.4
80000.0
SIDE ENTRY, EUCENTRIC
Data acquired using Leginon.
2013-04-26
GATAN ULTRASCAN 4000 (4k x 4k)
600
30
Each micrograph
C1
OTHER
16.0
OTHER
EMAN2, SPARX
60000