2021-11-24
REL
PDBj
PDBj
PDBj
2021-08-01
2021-11-24
2021-11-24
2021-11-24
Ministry of Science and Technology (MoST, Taiwan)
108-2320-B-001-011-MY3
Taiwan
Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex (conformation 3)
Li S
Hsieh K
Kuo C
Su S
Huang K
Zhang K
Chang CI
Li S
Hsieh KY
Kuo CI
Su SC
Huang KF
Zhang K
Chang CI
Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex.
Sci Adv
US
7
eabj9537
eabj9537
2021
34757797
doi:10.1126/sciadv.abj9537
2375-2548
EMD-31607
associated EM volume
Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex (conformation 3)
7fiz
FULLOVERLAP
Lon bound to endogenous substrate
Lon bound to endogenous substrate
0
1
2
Meiothermus taiwanensis WR-220
Escherichia coli
0.6
Lon protease
Meiothermus taiwanensis
0.090081336
6
Escherichia coli
LEVO
MRLELPVIPLRNTVILPHTTTPVDVGRAKSKRAVEEAMGADRLIFLVAQRDPEVDDPAPDDLYTWGVQAVVKQAMRLPDG
TLQVMVEARARAQVTDYIPGPYLRARGEVFSEIFPIDEAVVRVLVEELKEAFEKYVANHKSLRLDRYQLEAVKGTSDPAM
LADTIAYHATWTVAEKQEILELTDLEARLKKVLGLLSRDLERFELDKRVAQRVKEQMDTNQREYYLREQMKAIQKELGGE
DGLSDLEALRKKIEEVGMPEAVKTKALKELDRLERMQQGSPEATVARTYLDWLTEVPWSKADPEVLDINHTRQVLDEDHY
GLKDVKERILEYLAVRQLTQGLDVRNKAPILVLVGPPGVGKTSLGRSIARSMNRKFHRISLGGVRDEAEIRGHRRTYIGA
MPGKLIHAMKQVGVINPVILLDEIDKMSSDWRGDPASAMLEVLDPEQNNTFTDHYLDVPYDLSKVFFITTANTLQTIPRP
LLDRMEVIEIPGYTNMEKQAIARQYLWPKQVRESGMEGRIEVTDAAILRVISEYTREAGVRGLERELGKIARKGAKFWLE
GAWEGLRTIDASDIPTYLGIPRYRPDKAETEPQVGTAQGLAWTPVGGTLLTIEVAAVPGSGKLSLTGQLGEVMKESAQAA
LTYLRAHTQDYGLPEDFYNKVDLHVHVPDGATPKDGPSAGITMATAIASALSRRPARMDIAMTGEVSLRGKVMPIGGVKE
KLLAAHQAGIHKIVLPKDNEAQLEELPKEVLEGLEIKLVEDVGEVLEYLLLPEPTMPPVVQPSDNRQQPGAGAKLAAALE
HHHHHH
3.4.21.53
Unknown endogenous substrate
Meiothermus taiwanensis WR-220
0.001890321
1
Escherichia coli
LEVO
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
0.0005232469999999999
4
AGS
ADENOSINE-5'-DIPHOSPHATE
0.000427201
1
ADP
singleParticle
particle
7.5
Quantifoil R1.2/1.3
COPPER
200
GLOW DISCHARGE
ETHANE
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
FEI FALCON IV (4k x 4k)
48.0
1
C1
3.28
FSC 0.143 CUT-OFF
cryoSPARC
3.0
77159
MAXIMUM LIKELIHOOD
MAXIMUM LIKELIHOOD