RELPDBePDBePDBe2015-09-242015-10-142015-10-142016-03-30Bovine mitochondrial ATP synthase state 1aZhou ARohou ASchep DGBason JVMontgomery MGWalker JEGrigorieff NRubinstein JLATP synthase, rotary ATPaseZhou ARohou ASchep DGBason JVMontgomery MGWalker JEGrigorieff NRubinstein JLStructure and conformational states of the bovine mitochondrial ATP synthase by cryo-EMeLife4e10180201526439008doi:10.7554/eLife.101805araFULLOVERLAPBovine mitochondrial ATP synthaseBovine mitochondrial ATP synthaseDetergent-solubilized protein complexOne hetero-oligomeric ATP synthase complex10.6ATP synthaseBos taurusbovineHeartMitochondriaMitochondrial membrane0.61monomerfalsesingleParticleparticle87.220 mM Tris-HCl, 100 mM NaCl, 0.05% (wt/v) dodecylmaltoside, 2 mM ATP, 0.02% (wt/v) NaN3Home made holey carbon on 400 square mesh Cu/Rh grid, glow-discharged 2 minsETHANE-PROPANE MIXTURE100FEI VITROBOT MARK IIIBlot for 27 seconds before plungingFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.71.24.118000.030487.0FEI TITAN KRIOS AUTOGRID HOLDER80Objective lens astigmatism was corrected at 18,000x magnification (before detector post-magnification)K2 Summit direct detector device (Gatan Inc.) operated
in super-resolution mode with a 1.64 angstrom physical pixel and 0.82 angstrom super-resolution pixel.
With no specimen present, the rate of exposure of the detector was 8 electrons/pixel/second. Exposure-
fractionated movies of 20.1 s were recorded as stacks of 67 frames, so that selected
specimen areas were exposed with a total of 60.3 electrons/square angstrom.2015-03-15GATAN K2 (4k x 4k)5586760.38.FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.71.24.118000.030487.0FEI TITAN KRIOS AUTOGRID HOLDER80Objective lens astigmatism was corrected at 18,000x magnification (before detector post-magnification)K2 Summit direct detector device (Gatan Inc.) operated
in super-resolution mode with a 1.64 angstrom physical pixel and 0.82 angstrom super-resolution pixel.
With no specimen present, the rate of exposure of the detector was 8 electrons/pixel/second. Exposure-
fractionated movies of 20.1 s were recorded as stacks of 67 frames, so that selected
specimen areas were exposed with a total of 60.3 electrons/square angstrom.2014-09-28GATAN K2 (4k x 4k)5586760.38.The particles were selected using an automatic selection program.Each particleC1OTHER6.7OTHERRelion, FREALIGNTo avoid noise bias, only data up to a resolution of 10 angstrom were used during refinement.201042WSSABCDEFGHISFLEXIBLE FITChimera, MDFFRigid body fitting performed in Chimera first, followed by flexible fitting performed using Molecular Dynamics Flexible Fitting (MDFF).REAL2XNDJKLMNOPQFLEXIBLE FITChimera, MDFFRigid body fitting performed in Chimera first, followed by flexible fitting performed using Molecular Dynamics Flexible Fitting (MDFF).REAL2CLYDEFFLEXIBLE FITChimera, MDFFRigid body fitting performed in Chimera first, followed by flexible fitting performed using Molecular Dynamics Flexible Fitting (MDFF).REAL