RELPDBePDBePDBe2016-02-032016-03-092016-03-092016-03-09p97 in the ADP state no symmetry appliedSchuller JMBeck FLoessl PHeck Albert JRFoerster FAAA-ATPase / Cdc48 / Cryo-EM / ERAD / Protein quality controlSchuller JMBeck FLoessl PHeck Albert JRFoerster FNucleotide-dependent conformational changes of the AAA+ ATPase p97 revisitedFEBS LETT.Full-length human p97Full-length human p97p97/VCP Transitional endoplasmic reticulum ATPaseHomohexamer10.548491Native Mass-specp97/VCP Transitional endoplasmic reticulum ATPaseHomo sapiensHumanHomohexamertrueEscherichia coliM15pQE15P55072singleParticleparticle0.87.4Buffer: 40 mM Tris, 150 mM NaCl, 1 mM MgCl2Lacey Carbon gridsETHANE90120HOMEMADE PLUNGERBlot for 2 seconds before plungingFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.71.03.5105000.0FEI TITAN KRIOS AUTOGRID HOLDERObjective lens astigmatism was corrected at 105,000 times magnificationGIF Quantum0.020.02014-11-03GATAN K2 SUMMIT (4k x 4k)327640Every image is the average of 30 frames recorded by the direct electron detectorThe particles were selected using an automatic selection program implemented in the TOM toolbox.On the micrograph levelC1OTHER9.0OTHERRELIONMap was corrected using a B-factor of -571 A^2.40000