REL
PDBe
PDBe
PDBe
2016-03-09
2016-04-13
2016-05-18
2016-06-15
Transcription initiation complex structures elucidate DNA opening (OC5)
Plaschka C
Hantsche M
Dienemann C
Burzinski C
Plitzko J
Cramer P
Gene expression, Transcription initiation
Plaschka C
Hantsche M
Dienemann C
Burzinski C
Plitzko J
Cramer P
Transcription initiation complex structures elucidate DNA opening
NATURE
533
353
358
2016
27193681
doi:10.1038/NATURE17990
yeast Pol II transcription initiation complex (open DNA)
yeast Pol II transcription initiation complex (open DNA)
Monomer
7
0.89
DNA-directed RNA polymerase II
Saccharomyces cerevisiae
BJ5464 Rpb3 His-Bio
Baker's yeast
0.513
1
false
Transcription Factor IIA
Saccharomyces cerevisiae
Baker's yeast
0.045
1
true
Escherichia coli
BL21(DE3)RIL
pOPINE
Transcription Factor IIB
Saccharomyces cerevisiae
Baker's yeast
0.038
1
true
Escherichia coli
BL21(DE3)RIL
pET21b
TATA-box-binding protein
Saccharomyces cerevisiae
Baker's yeast
0.027
1
true
Escherichia coli
BL21(DE3)RIL
pOPINE
Transcription Factor IIE
Saccharomyces cerevisiae
Baker's yeast
0.091
1
true
Escherichia coli
BL21(DE3)RIL
pET21
Transcription Factor IIF
Saccharomyces cerevisiae
Baker's yeast
0.129
1
true
Escherichia coli
BL21(DE3)RIL
pETDuet-1
synthetic promoter DNA construct
unidentified
0.044
closed promoter DNA was opened by transcription machinery
DNA
DOUBLE HELIX
true
singleParticle
particle
0.3
7.5
25 mM HEPES-KOH pH 7.5, 150 mM potassium acetate, 2 mM MgCl2, 5 mM DTT
R3.5/1 holey carbon grids (Quantifoil)
ETHANE
100
FEI VITROBOT MARK IV
Quantifoil R 3.5/1 holey carbon grids were glow-discharged before deposition of 4.5 microliters of sample. Grids were then blotted for 8.5 s and plunge-frozen in liquid ethane.
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
0.8
5.0
37000.0
37037.0
FEI TITAN KRIOS AUTOGRID HOLDER
GIF Quantum
0.0
20.0
2016-06-20
GATAN K2 SUMMIT (4k x 4k)
959
40
Each particle
C1
OTHER
6.1
OTHER
RELION
79797