RELPDBePDBePDBe2016-05-252016-07-272016-07-272016-07-27RNA polymerase I-Rrn3 complex at 4.8 A resolutionEngel CPlitzko JCramer PRNA polymerase, transciptionEngel CPlitzko JCramer PRNA polymerase I-Rrn3 complex at 4.8 A resolutionNAT.COMMUN.712129201627418309doi:10.1038/NCOMMS121295g5lFULLOVERLAPS. cerevisiae RNA polymerase I bound to the initiation factor Rrn3S. cerevisiae RNA polymerase I bound to the initiation factor Rrn3monomeric20.6630.663Size exclusion chromatographyRNA polymerase ISaccharomyces cerevisiaeCB010Bakers yeastNucleus0.590.591monomerfalseGO:0005736RNA polymerase I-specific transcription initiation factor RRN3Saccharomyces cerevisiaeCB010Bakers yeastNucleus0.0730.07311trueEscherichia coliBL21(DE3)RILpET28P36070IPR007991singleParticleparticle0.17.8150mM NaCl, 5mM Hepes, 1mM MgCl2, 0.01 mM ZnCl2, 5 mM DTTNEGATIVEno stainingR2/1 holey carbon grids (Quantifoil)ETHANE100FEI VITROBOT MARK IV4.5 microliters of sample was applied to glow-discharged Quantifoil R 2/1 holey carbon grids, which were then blotted for 8.5s and plunge-frozen in liquid ethaneFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.70.83.637000.037037.0FEI TITAN KRIOS AUTOGRID HOLDER0GIF Quantum0.020.02015-03-27GATAN K2 SUMMIT (4k x 4k)117440Movies with 33 frames were collected over 9.9 secondseach particleC1OTHER4.8OTHERRELION634454C2MABCDEFGHIJKLMNRIGID BODY FITChimera, COOTInitial placement was performed in Chimera. Domains as specified in the PDB file were fitted as rigid bodies an hinges regularized in COOT.REAL165.03TJ1ARIGID BODY FITChimera, COOTAfter initial placement in Chimera, secondary structure elements were rigid body fittet in COOT and hinges regularized.REAL165.0