2018-12-05RELPDBePDBePDBe2018-03-122016-08-242018-12-052018-12-05Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (Map B)Llacer JLHussain TGordiyenko YRamakrishnan VLlacer JLHussain TSaini AKNanda JDKaur SGordiyenko YKumar RHinnebusch AGLorsch JRRamakrishnan VTranslational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition.ElifeUS7201830475211doi:10.7554/eLife.392732050-084XEMD-4329other EM volumeEMD-4330other EM volumeEMD-4331associated EM volumeStructure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model B)Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model B)01234567891011121314151617181920212223242526272829303132333435363738394041424344454647Kluyveromyces lactis1.8singleParticleparticle0.156.520.0MES5.0Magnessium acetate80.0Potassium acetate10.0Ammonium acetate2.0Dithiothreitol (DTT)0.001Zinc acetate0.6ATP0.25GDPCPQuantifoil R2/2COPPER400CARBONCONTINUOUSGLOW DISCHARGEETHANE100277FEI VITROBOT MARK IFEI POLARA 300FLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30050.02.01.53.578000.104478.NITROGEN90.0100.0FEI FALCON III (4k x 4k)INTEGRATING336001.140.0Images were collected in movie-mode at 32 frames per second1FEI Falcon III706713Gctf4V88C1FOURIER SPACE3.5FSC 0.143 CUT-OFFRELION1.454699NOT APPLICABLERELION1.4ANGULAR RECONSTITUTIONRELION1.4RELION1.4OTHERFSCRECIPROCAL54.emd_4331_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
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