2019-12-18RELPDBePDBePDBe2018-12-092019-06-192019-06-192019-12-18Swedish Research Council628-20081109822-2010-6157, 822-2012-5260 and 828-2012-108SwedenEuropean Research CouncilERC-291602SwedenKnut and Alice Wallenberg FoundationKAW-2011.081SwedenHigh resolution electron cryo-microscopy structure of the bacteriophage PR772Narayana Reddy HKSvenda MReddy HKHajdu JCarroni MSvenda MElectron cryo-microscopy of Bacteriophage PR772 reveals the elusive vertex complex and the capsid architecture.ElifeUS8201931513011doi:10.7554/eLife.484962050-084XNarayana Reddy HKHajdu JCarroni MSvenda MElectron cryo-microscopy of Bacteriophage PR772 reveals the composition and structure of the elusive vertex complex and the capsid architectureBiorxivUS2019doi:10.1101/645523EMD-4461associated EM volumeHigh resolution electron cryo-microscopy structure of the bacteriophage PR7726q5uFULLOVERLAPBacteriophage PR772Bacteriophage PR772012345Wild typeEnterobacteria phage PR77286Major Capsid Protein (P3)Enterobacteria phage PR7720.04350549212LEVOMAQVQQLTPAQQAALRNQQAMAANLQARQIVLQQSYPVIQQVETQTFDPANRSVFDVTPANVGIVKGFLVKVTAAIKNNH
ATEAVALTDFGPANLVQRVIYYDPDNQRHTETSGWHLHFVNTAKQGAPFLSSMVTDSPIKYGDVMNVIDAPATIAAGATG
ELTMYYWVPLAYSETDLTGAVLANVPQSKQRLKLEFANNNTAFAAVGANPLEAIYQGAGAADCEFEEISYTVYQSYLDQL
PVGQNGYILPLIDLSTLYNLENSAQAGLTPNVDFVVQYANLYRYLSTIAVFDNGGSFNAGTDINYLSQRTANFSDTRKLD
PKTWAAQTRRRIATDFPKGVYYCDNRDKPIYTLQYGNVGFVVNPKTVNQNARLLMGYEYFTSRTELVNAGTISTTMinor Capsid Protein (P30)Enterobacteria phage PR7720.0092755591LEVOMALINPQFPYAGPVPIPGPAPTETMPLLNYRVEGRIAGIQQARQFMPFLQGPHREVAEQTYYAIGTGIQMGQTFNQPLIN
TQEGInfectivity Protein (P16)Enterobacteria phage PR7720.0126162621LEVOMDKKKLLYWVGGGLVLIIIWLWFRNRPAAQVASNWEGPPYMTYNQPQAGSVTLPVAGYTSPSLTLPNRNRSCGCNPAVSA
AMAQGADLASKLTESISSQLNNYAESLNDYLASQAGVSpike proteinEnterobacteria phage PR7720.0344838983LEVOMANQQIGGSTVTYNGAIPMGGPVAINSVIEIAGTEVLVDLKLDYATGKISGVQTLYIDLRDFLGDVTVTMPDTGQRITAR
AGTQGYYPVLSTNLMKFIVSATIDGKFPMNFINFPIALGVWPSGIKGDKGDPGAPGPAGGTVVVEDSGASFGESLLDTTS
EPGKILVKRISGGSGITVTDYGDEVEIEASGGGGGGGGVTDALSLMYSTSTGGPASIAANALTDFDLSGALTVNTVGTGL
TKSAAGIQLAAGKSGLYQITMTVKNNTVTTGNYLLRVKYGSSDFVVACPASSLTAGGTISLLIYCDVLGVPSLDVLKFSL
CNDGAALSNYIINITAAKINPenton proteinEnterobacteria phage PR7720.0137573882LEVOMNVNNPNQMTVTPVYNGCDSGEGPQSVRGYFDAVAGENVKYDLTYLADTQGFTGVQCIYIDNAENDGAFEIDVEETGQRI
KCPAGKQGYFPLLVPGRAKFVARHLGSGKKSVPLFFLNFTIAQGVWsingleParticleparticle78C-flat-2/2COPPERGLOW DISCHARGEAIRETHANE100298.15FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.70.82.6130000.0NITROGENGATAN K2 SUMMIT (4k x 4k)3-401320040.0156000I2.75FSC 0.143 CUT-OFF46000MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODAB INITIO MODELCoot0.8.9.1Cross-correlation coefficientREAL104.93