2019-05-15RELPDBePDBePDBe2019-01-032019-05-152019-05-152019-05-15Human post-catalytic spliceosome (P complex) stalled with DHX8 S717A mutant, focused refinement on coreFica SMOubridge CWilkinson MENewman AJNagai KNFica SMOubridge CWilkinson MENewman AJNagai KA human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.ScienceUS363710714201930705154doi:10.1126/science.aaw55691095-92030038SCIEASEMD-4526other EM volumeEMD-4528associated EM volumeHuman post-catalytic P complex spliceosomeHuman post-catalytic P complex spliceosome01234567891011121314151617181920212223242526272829303132333435363738394041424344Homo sapiens3.0singleParticleparticle17.920.0HEPES100.0KCl0.2EDTAETHANE100277FEI VITROBOT MARK III3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane.. FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.71.53.5130000.GATAN K2 SUMMIT (4k x 4k)COUNTING1-281396014.066.01C12.9FSC 0.143 CUT-OFFRELION3.0132996MAXIMUM LIKELIHOODRELION3.0MAXIMUM LIKELIHOODRELION3.0