2019-11-06RELPDBePDBePDBe2019-03-222019-05-082019-05-082019-11-06Swiss National Science Foundation150665SwitzerlandStructure of a membrane adenylyl cyclase bound to an activated stimulatory G protein (SOL-C map)Korkhov VMQi CQi CSorrentino SMedalia OKorkhov VMThe structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein.ScienceUS364389394201931023924doi:10.1126/science.aav07781095-92030038SCIEASEMD-4719other EM volumeComplete AC9-GalphaS complexEMD-4722other EM volumeSoluble domain of AC9-GalphaS complexEMD-4724associated EM volumeComplex of adenylyl cyclase AC9 with G protein subunit GalphasComplex of adenylyl cyclase AC9 with G protein subunit Galphas012Adenylate cyclase 911Bos taurusHomo sapiensHEK293FpEZT-BMGuanine nucleotide-binding protein G(s) subunit alpha isoforms short12Bos taurusTrichoplusia niHigh FivepFastbacAdenylyl cylcase AC9Bos taurusHomo sapiensLEVOMASPPHQQLLQHHSTEVSCDSSGDSNSVRVRINPKQPSSNSHPKHCKYSISSSCSSSGDSGGVPRRMGAGGRLRRRKKLPQLFERASSRWWDPKFDSVNLEEACMERCFPQTQRRFRYALFYIGFACLLWSIYFGVHMKSKLIVMVAPALCFLVVCVGFFLFTFTKLYARHYVWTSLVLTLLVFALTLAAQFQVLTPLSGRVDNFNHTRAARPTDTCLSQVGSFSMCIEVLFLLYTVMHLPLYLSLILGVAYSVLFETFGYHFQDEACFASPGAEALHWELLSRALLHLCIHAIGIHLFIMSQVRSRSTFLKVGQSIMHGKDLEVEKALKERMIHSVMPRIIADDLMKQGDEESENSVKRHATSSPKNRKKKSSIQKAPIAFRPFKMQQIEEVSILFADIVGFTKMSANKSAHALVGLLNDLFGRFDRLCEETKCEKISTLGDCYYCVAGCPEPRADHAYCCIEMGLGMIRAIEQFCQEKKEMVNMRVGVHTGTVLCGILGMRRFKFDVWSNDVNLANLMEQLGVAGKVHISEATAKYLDDRYEMEDGKVTERLGQSVVADQLKGLKTYLIAGQRAKESHCSCSEALLSGFEVLDGSRVSSGPRGQGTASPGSVSDLAQTVKTFDNLKTCPSCGITFTPKPEAGAEGGAVQNGCQEEPKNSAKASGGPSSKTQNGLLSPPPEEKLTNSQTSLCEILQEKGRWAGVSLDQSALLPLRFKNIREKTDAHFVDVIKEDSLMKDYFFKPPINQFSLNFLDPELERAYRTSYQEEVVKSSPVRTFASATFSSLLDVLLSTTVFLILSITCFLRYGAASTPPPPAALAVFGAALLLEILSLVVSVRMVFFLEDVMTCTKRLLEWIAGWLPRHFIGAILVSLPALAVYSHVTSEFETNIHSTMFTGSAVLTAVVQYCNFCQLSSWMRSSLATVVGAGPLLLLLYVSLCPDSSTVISHLDAVQNFSSTRKLCNASLPHDGRSPASLIGQEVILVFFLLLLLVWFLNREFEVSYRLHYHGDVEADLHRTKIQSMRDQADWLLRNIIPYHVAEQLKVSQTYSKNHDSGGVIFASIVNFSEFYEENYEGGKECYRVLNELIGDFDELLSKPDYSSIEKIKTIGATYMAASGLNATQCRDGSHPQEHLQILFEFAKEMMRVVDDFNNNMLWFNFKLRVGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDTTGVECRIQVSEESYRVLSKMGYEFDYRGTVNVKGKGQMKTYLYPKCTDSGLVPQHQLSISPDIRVQVDGSIGRSPTDEIASLVPSVQNPDQVPPGSENNAQTRDAHPSAKRPWKEPVRAEERCRFGKAIEKSDCEEVGMEEANELTKLNVSERAAAALEVLFQGPGGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKAASAWSHPQFEKGGGSGGGSGGSAWSHPQFEK4.6.1.1Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortBos taurusTrichoplusia niLEVOMGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGGEGGEEDPNAKSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELLGGHHHHHHHHsingleParticleparticle8.0ETHANE100278FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3000.752.5FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 QUANTUM (4k x 4k)SUPER-RESOLUTION58178.047.01656817GctfInitial model generated using cryoSPARCC13.6FSC 0.143 CUT-OFFRELION2.1.0Continued refinement using a mask excluding the membrane portion of the AC9 and the G protein16343MAXIMUM LIKELIHOODRELION2.1.0MAXIMUM LIKELIHOODRELION2.1.03RELION2.1.0AB INITIO MODELCross-correlation coefficientREAL149.610000000000014