2020-12-02RELPDBePDBePDBe2019-03-292019-08-072019-08-072020-12-02German Research FoundationKE1879/3-1GermanyEuropean Research CouncilCRYOTRANSLATIONGerman Research FoundationCRC 1208GermanyRibosome-bound SecYEG translocon in a nanodiscKater LBeckmann RKedrov AKater LFrieg BBerninghausen OGohlke HBeckmann RKedrov APartially inserted nascent chain unzips the lateral gate of the Sec translocon.Embo Rep.UK20e48191e48191201931379073doi:10.15252/embr.2019481911469-3178EMD-4743associated EM volumeRbibosome-bound SecYEG translocon in a nanodisc6r7lFULLOVERLAPStalled E. coli ribosome nascent chain complex (RNC) bound to the translocon SecYEG in a nanodiscStalled E. coli ribosome nascent chain complex (RNC) bound to the translocon SecYEG in a nanodisc0123Escherichia coliSecGEscherichia coli0.0018903211LEVO(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)SecE,Protein translocase subunit SecE,Protein translocase subunit SecE,Protein translocase subunit SecEEscherichia coli0.0108801221LEVO(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)LLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTAVM
SLILWGLDGILVRLVSFITGLRFProtein translocase subunit SecYEscherichia coli0.0485533751LEVOMAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGIDAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFAL
GIMPYISASIIIQLLTVVHPTLAEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFLVLLLVAVLVFAVTFFVVFVERG
QRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIPAIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLL
YASAIIFFCFFYTALVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMRDAMKVPFY
FGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGRsingleParticleparticle7.4ETHANEFEI TITAN KRIOSOTHERBRIGHT FIELDFIELD EMISSION GUN300FEI FALCON II (4k x 4k)INTEGRATING40964096130982.51837184Gctf1.06C1FOURIER SPACE6.0FSC 0.143 CUT-OFFRELION3.0112366MAXIMUM LIKELIHOODRELION3.0MAXIMUM LIKELIHOODRELION3.0emd_4743_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
450
450450
0
00450450450975.6975.6975.690.090.090.0XYZ-0.34070330.62183416-0.000229804910.0100340172.1682.1682.168Large ribosomal subunit portion of the RNC:SecYEG:Nanodisc complex after multi-body refinement. This map includes the tRNA, nascent chain, SecYEG and nanodisc. Filtered according to local resolution.emd_4743_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
450
450450
0
00450450450975.6975.6975.690.090.090.0XYZ-0.251200650.55576134-0.000200448470.012850292.1682.1682.168Large ribosomal subunit portion of the RNC:SecYEG:Nanodisc complex after multi-body refinement. This map includes the tRNA, nascent chain, SecYEG and nanodisc. Filtered according to local resolution.