2020-01-22RELPDBePDBePDBe2019-04-012019-07-102019-07-102020-01-22German Research FoundationExcellence Clusters CIPSMGermanyGerman Research FoundationSFB 1035GermanySubtomogram average of cytosolic ribosomes after cryo-FIB lift-out (p1 state)Pfeffer SMahamid JAlbert SPlitzko JMSchaffer MPfeffer SMahamid JKleindiek SLaugks TAlbert SEngel BDRummel ASmith AJBaumeister WPlitzko JMA cryo-FIB lift-out technique enables molecular-resolution cryo-ET within native Caenorhabditis elegans tissue.Nat.MethodsUS16757762201931363205doi:10.1038/s41592-019-0497-51548-7105EMD-4758other EM volumep2 stateEMD-4756other EM volumep1-pre stateEMD-4755other EM volumep1-post stateEMD-4757associated EM volumeCytosolic ribosome in the native cellular environment after cryo-FIB lift-out from C. elegans (p1 state)Cytosolic ribosome in the native cellular environment after cryo-FIB lift-out from C. elegans (p1 state)0Caenorhabditis eleganssubtomogramAveragingtissue7.3NITROGENThe sample was high pressure frozen in a BAL-TEC HPM 100 high-pressure freezer.. Adult C. elegans worms.FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)COUNTING1.51C111.8FSC 0.143 CUT-OFFRELION4895513688IMODPyTomRELIONMAXIMUM LIKELIHOODRELION