2019-12-18RELPDBePDBePDBe2019-04-022019-05-222019-06-122019-12-18Swiss National Science FoundationCRSII3_160734/1SwitzerlandEuropean Commission667951European Union666068Japan Society for the Promotion of ScienceJP18H05534JapanEuropean Commission705354Japan Agency for Medical Research and Development (AMED)JP18am0101076JapanJapan Society for the Promotion of ScienceJP16H06307JapanCryo-EM structure of NCP-6-4PPMatsumoto SCavadini SBunker RDThoma NHMatsumoto SCavadini SBunker RDGrand RSPotenza ARabl JYamamoto JSchenk ADSchubeler DIwai SSugasawa KKurumizaka HThoma NHDNA damage detection in nucleosomes involves DNA register shifting.NatureUK5717984201931142837doi:10.1038/s41586-019-1259-31476-46870006NATUASEMD-4767associated EM volumeCryo-EM structure of NCP-6-4PP6r93FULLOVERLAP6-4PP nucleosome6-4PP nucleosome01234560.199DNA112Homo sapienssynthetic constructHistone H3.1, Histone H2A, Histone H2B1356Homo sapiensEscherichia coli BL21(DE3)Histone H414Homo sapiensEscherichia coliHuman alpha-satellite DNA (145-MER)Homo sapiens0.0447656641(DA)(DT)(DC)(DA)(DA)(DT)(DA)(DT)(DC)(DC)(DA)(DC)(DC)(DT)(DG)(DC)(DA)(DG)(DA)(DT)
(DT)(DC)(DT)(DA)(DC)(DC)(DA)(DA)(DA)(DA)(DG)(DT)(DG)(DT)(DA)(DT)(DT)(DT)(DG)(DG)
(DA)(DA)(DA)(DC)(DT)(DG)(DC)(DT)(DC)(DA)(DA)(DC)(DC)(DA)(DA)(DA)(DA)(DG)(DG)(DC)
(DA)(DT)(DG)(DT)(DT)(DC)(DA)(DG)(DC)(DT)(DG)(DG)(DT)(DT)(DC)(DA)(DG)(DC)(DT)(DG)
(DA)(DA)(DC)(DA)(DT)(DG)(DC)(DC)(DT)(DT)(DT)(DT)(DG)(DA)(DT)(DG)(DG)(DA)(DG)(DC)
(DA)(DG)(DT)(DT)(DT)(DC)(DC)(DA)(DA)(DA)(DT)(DA)(DC)(DA)(DC)(DT)(DT)(DT)(DT)(DG)
(DG)(DT)(DA)(DG)(DA)(DA)(DT)(DC)(DT)(DG)(DC)(DA)(DG)(DG)(DT)(DG)(DG)(DA)(DT)(DA)
(DT)(DT)(DG)(DA)(DT)DNAHuman alpha-satellite DNA (145-MER) with a 6-4PP at positions 95-96Homo sapiens0.045038852145 bp human alpha-satellite with a 6-4 pyrimidine-pyrimidone (6-4PP) at base positions 95-96.1(DA)(DT)(DC)(DA)(DA)(DT)(DA)(DT)(DC)(DC)(DA)(DC)(DC)(DT)(DG)(DC)(DA)(DG)(DA)(DT)
(DT)(DC)(DT)(DA)(DC)(DC)(DA)(DA)(DA)(DA)(DG)(DT)(DG)(DT)(DA)(DT)(DT)(DT)(DG)(DG)
(DA)(DA)(DA)(DC)(DT)(DG)(DC)(DT)(DC)(DC)(DA)(DT)(DC)(DA)(DA)(DA)(DA)(DG)(DG)(DC)
(DA)(DT)(DG)(DT)(DT)(DC)(DA)(DG)(DC)(DT)(DG)(DA)(DA)(DC)(DC)(DA)(DG)(DC)(DT)(DG)
(DA)(DA)(DC)(DA)(DT)(DG)(DC)(DC)(DT)(DT)(DT)(DT)(DG)(DA)(T64)(DG)(DG)(DA)(DG)
(DC)(DA)(DG)(DT)(DT)(DT)(DC)(DC)(DA)(DA)(DA)(DT)(DA)(DC)(DA)(DC)(DT)(DT)(DT)(DT)
(DG)(DG)(DT)(DA)(DG)(DA)(DA)(DT)(DC)(DT)(DG)(DC)(DA)(DG)(DG)(DT)(DG)(DG)(DA)(DT)
(DA)(DT)(DT)(DG)(DA)(DT)DNAHistone H3.1Homo sapiens0.0157194452Escherichia coli BL21(DE3)LEVOGSHMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQ
DFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERAHistone H4Homo sapiens0.0116767032Escherichia coliLEVOGSHMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHA
KRKTVTAMDVVYALKRQGRTLYGFGGHistone H2A type 1-B/EHomo sapiens0.014447825GSHMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK2Escherichia coli BL21(DE3)LEVOGSHMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKT
RIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGKHistone H2B type 1-JHomo sapiens0.0142175162Escherichia coli BL21(DE3)LEVOGSHMPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGE
ASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAKsingleParticleparticle27.450.0NaClSodium chloride50.0HEPES0.25TCEPETHANE85277LEICA EM GPFEI TITAN KRIOSSPOT SCANBRIGHT FIELDFIELD EMISSION GUN30050.00.0130000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGIF Quantum LS20GATAN K2 SUMMIT (4k x 4k)COUNTING16.040.01Gctf4.0FSC 0.143 CUT-OFFRELION298378OTHERRELIONOTHERRELION4ZUXI4ZUXJ5Y0CA5Y0CB5Y0CC5Y0CD5Y0CE5Y0CF5Y0CG5Y0CH4E54B3EI4AFLEXIBLE FITCross-correlation coefficientREAL