REL
RCSB
RCSB
RCSB
2011-05-02
2011-05-05
2012-03-08
2012-10-03
Poliovirus 135S particle and P1 Fab complex at 12-angs. resolution
Lin J
Cheng N
Chow M
Filman DJ
Steven AC
Hogle JM
Belnap DM
picornavirus, viral cell entry, viral uncoating, virus-antibody complex, virus-Fab complex, virus disassembly, virus uncoating, virus conformational transitions, monospecific antibody
Lin J
Cheng N
Chow M
Filman DJ
Steven AC
Hogle JM
Belnap DM
An externalized polypeptide partitions between two distinct sites on genome-released poliovirus particles.
J.VIROL.
85
9974
9983
2011
21775460
doi:10.1128/JVI.05013-11
Poliovirus 135S particle and P1(monospecific antibody) Fab complex
Poliovirus 135S particle and P1(monospecific antibody) Fab complex
135S particle icosahedral with Fab
2
Human poliovirus 1 Mahoney
native virus 160S is converted by heat-treatment to 135S
Human poliovirus 1 Mahoney
Homo sapiens
VERTEBRATES
340
1
VIRION
STRAIN
false
false
poliovirus 135S
singleParticle
particle
7.5
20 mM Tris, 2 mM CaCl2
ETHANE
OTHER
Vitrification carried out in ambient atmosphere. Ethane cooled by liquid nitrogen.
Blotted manually before plunging
FEI/PHILIPS CM200FEG
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
120
2
0.81
2.24
38000.0
37752.0
GATAN LIQUID NITROGEN
Bsoft
1999-07-15
KODAK SO-163 FILM
ZEISS SCAI
7
14
10
Defocal pairs were used.
8.
Side entry liquid nitrogen-cooled cryo specimen holder
0
0
CTF and decay correction of each particle
OTHER
12.0
FSC 0.5 CUT-OFF
EM3DR2
Reconstruction computed from focal pairs. Pairs not summed for reconstruction calculation.
10160