RELRCSBRCSBRCSB2014-01-202014-02-192014-02-192016-01-27Electron cryo-microscopy of nanobody AB6 in complex with poliovirus P1/MahoneySchotte LStrauss MThys BHalewyck HFilman DJBostina MHogle JMRombaut Bpicornavirus, nanobody, antibody, VHH, poliovirus, mechanism of neutralizationSchotte LStrauss MThys BHalewyck HFilman DJBostina MHogle JMRombaut BMechanism of action and capsid stabilizing properties of VHHs with an in vitro anti-polioviral activityJ.VIROL.8844034413201424501405doi:10.1128/JVI.03402-13Strauss MSchotte LThys BFilman DJHogle JMFive of five VHHs neutralizing poliovirus bind the receptor-binding site.J.VIROL.90201626764003doi:10.1128/JVI.03017-15EMD-5888FULLOVERLAP3jbdFULLOVERLAPnanobody AB6 in complex with poliovirus P1/Mahoneynanobody AB6 in complex with poliovirus P1/Mahoney160 nanobody VHH monomers bind to each poliovirion29.09.01Human poliovirus 1Human poliovirus 1Homo sapiensVERTEBRATES993501VIRIONSEROTYPEfalsefalseMahoneynanobody PVSP6ACamelus dromedariusdromedaryEach virus is decorated with 60 copies of nanobody PVSP6A.60trueEscherichia coliWK6pHEN6(c)singleParticleparticle17.4145 mM NaCl, 50 mM Na2HPO4.12H2OQuantifoil R2/2ETHANE154HOMEMADE PLUNGER2 second blotFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN2002.61.03.096000.089232.0FEI TITAN KRIOS AUTOGRID HOLDER2012-10-17GATAN ULTRASCAN 4000 (4k x 4k)1562232516.The particles were processed using Frealign.per particle4.8OTHERFrealign572821HXSRIGID BODY FITREFMAC5, COOT, SPDBVRigid body with some flexible loops and side chains. LSQKAB was applied after each refinement cycle to re-impose exact icosahedral operators and rigid body constraints.Stereochemically restrained maximum likelihood refinement of both Fourier phase and amplitude agreementRECIPROCAL2PLVRIGID BODY FITREFMAC5, COOT, SPDBVRigid body with some flexible loops and side chains. LSQKAB was applied after each refinement cycle to re-impose exact icosahedral operators and rigid body constraints.Stereochemically restrained maximum likelihood refinement of both Fourier phase and amplitude agreementRECIPROCAL4BELRIGID BODY FITREFMAC5, COOT, SPDBVRigid body with some flexible loops and side chains. LSQKAB was applied after each refinement cycle to re-impose exact icosahedral operators and rigid body constraints.Stereochemically restrained maximum likelihood refinement of both Fourier phase and amplitude agreementRECIPROCAL2X1PRIGID BODY FITREFMAC5, COOT, SPDBVRigid body with some flexible loops and side chains. LSQKAB was applied after each refinement cycle to re-impose exact icosahedral operators and rigid body constraints.Stereochemically restrained maximum likelihood refinement of both Fourier phase and amplitude agreementRECIPROCAL