RELRCSBRCSBRCSB2014-03-192014-04-092014-04-092014-04-30Kinetic and Structural Analysis of Coxsackievirus B3 Receptor Interactions and Formation of the A-particleOrgantini LJMakhov AMConway JFHafenstein SCarson SDcoxsackievirus b3, cvb3, CAR, cryoEM, A-particleOrgantini LJMakhov AMConway JFHafenstein SCarson SDKinetic and structural analysis of coxsackievirus B3 receptor interactions and formation of the A-particle.J.VIROL.8857555765201424623425doi:10.1128/JVI.00299-143j6lFULLOVERLAP3j6mFULLOVERLAP3j6nFULLOVERLAP3j6oFULLOVERLAPCoxsackievirus B3 complexed with CARCoxsackievirus B3 complexed with CARpurified virus and receptor complex in solutionicosahedral virus27Human coxsackievirus B3Virus was incubated with excess CAR at 4 degrees C.Human coxsackievirus B3CVB3/28Homo sapiensVERTEBRATES7VP1-43001VIRIONSTRAINfalsefalseCVB3Coxsackievirus and adenovirus receptorHomo sapienshumantrueEscherichia coliP78310singleParticleparticle0.16.050 mM MES, 100 mM NaClglow-discharged holey carbon Quantifoil electron microscopy gridsETHANE-PROPANE MIXTURE9595FEI VITROBOT MARK IIIFEI TECNAI F20SPOT SCANBRIGHT FIELDFIELD EMISSION GUN20021.983.6650000.050000.0GATAN LIQUID NITROGENCTFFIND32012-08-01KODAK SO-163 FILMNIKON SUPER COOLSCAN 90006.359615Particles were selected using EMAN.AUTO3DEMOTHER9.0OTHEREMAN, AUTO3DEM93021F5WBRIGID BODY FITSitusThe four available CAR structures were fit into the receptor density separately.correlation coefficientREAL1KACBRIGID BODY FITSitusThe four available CAR structures were fit into the receptor density separately.correlation coefficientREAL3JZ7KRIGID BODY FITSitusThe four available CAR structures were fit into the receptor density separately.correlation coefficientREAL3MJ7SRIGID BODY FITSitusThe four available CAR structures were fit into the receptor density separately.correlation coefficientREAL