RELRCSBRCSBRCSB2014-10-252014-11-192014-11-192014-12-10Structures of Protective Antibodies Reveal Sites of Vulnerability on Ebola VirusMurin CDFusco MLBornholdt ZAQiu XOlinger GGZeitlin LKobinger GPWard ABSaphire EOEbola, Antibody cocktails, mAbs, ZMAb, ZMapp, MB-003Murin CDFusco MLBornholdt ZAQiu XOlinger GGZeitlin LKobinger GPWard ABSaphire EOStructures of Protective Antibodies Reveal Sites of Vulnerability on Ebola VirusPROC.NAT.ACAD.SCI.USA1111718217187201425404321doi:10.1073/pnas.1414164111Fab fragment of c13C6 and c4G7 chimerized human monoclonal IgG1 antibody bound to Ebola virus glycoproteinFab fragment of c13C6 and c4G7 chimerized human monoclonal IgG1 antibody bound to Ebola virus glycoproteinSix Fab fragments bound to a single, trimeric GP (two Fabs per protomer)30.75Ebola virus glycoproteinEbola virus sp.0.451trimertrueDrosophila melanogasterSchneider 2 (S2)pMT-purochimerized human IgG1 antigen binding fragment c13C6Homo sapienshuman3monomertrueNicotiana benthamianachimerized human IgG1 antigen binding fragment c4G7Homo sapienshuman3monomertrueNicotiana benthamianasingleParticleparticle0.037.420 mM Tris, 150 mM NaClNEGATIVETo prepare negative stain grids, a 4 uL aliquot of each complex, which had been diluted to a concentration of ~0.03 ug/mL with TBS buffer, was placed for 15 seconds onto carbon-coated 400 Cu mesh grids that had been plasma cleaned for 20 s (Gatan), blotted off on the edge of the grid, then immediately stained for 30 s with 4 uL of 2% uranyl formate. The stain was blotted off on the edge of the grid and the grid was allowed to dry.400 Cu meshNONEOTHERFEI TECNAI F20FLOOD BEAMBRIGHT FIELDTUNGSTEN HAIRPIN1201.01.052000.0OTHER2014-02-05OTHER6524.0OTHEREMAN2, XMipp, IMAGIC10210