RELRCSBRCSBRCSB2014-11-192014-12-242015-01-212015-01-21Negative stain electron microscopy of JRFL SOSIP liganded with VRC01Guenaga Jde Val NWard ABWyatt RTGuenaga Jde Val NTran KFeng YSatchwell KWard ABWyatt RTWell-ordered Trimeric HIV-1 Subtype B and C Soluble Spike Mimetics Generated by Negative Selection Display Native-like PropertiesPLOS Pathogens11e1004570e1004570201525569572doi:10.1371/journal.ppat.1004570JRFL SOSIP liganded with VRC01JRFL SOSIP liganded with VRC01One trimer of JRFL SOSIP binds 3 VRC01 molecules20.5700.570Size exclusion chromatography (SEC)JRFL SOSIP gp140Simian-Human immunodeficiency virus0.5700.5701trimertrueHomo sapiensHEK 293FVRC01unidentified3monomerfalsesingleParticleparticle0.57.450 mM Tris-HCl, 150 mM NaClNEGATIVEGrids were stained for 30 seconds with 2% uranyl formate.400 Cu mesh grids, glow-discharged at 15 mA for 30 secondsNONEOTHERFEI TECNAI SPIRITFLOOD BEAMBRIGHT FIELDLAB61200.751.046000.052000.0OTHER2014-03-26TVIPS TEMCAM-F416 (4k x 4k)19229.2804020.0OTHEREMAN2, sparx227623J5MRIGID BODY FITREAL