RELRCSBRCSBRCSB2014-12-052015-01-282015-01-282015-02-11Structure of ADP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopyZhao MWu SZhou QVivona SCipriano DJCheng YBrunger ATATPases associated with diverse cellular activitiesZhao MWu SZhou QVivona SCipriano DJCheng YBrunger ATMechanistic insights into the recycling machine of the SNARE complexNATURE5186167201525581794doi:10.1038/nature141483j95FULLOVERLAPADP-bound N-ethylmaleimide sensitive factorADP-bound N-ethylmaleimide sensitive factorhexamer10.5N-ethylmaleimide sensitive factorCricetulus griseusChinese hamster0.0836hexamertrueEscherichia coliBL21(DE3)-RILpPROEX-1P18708singleParticleparticle158.050 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA, 1 mM ATP, 1 mM DTT, 0.05% v/v Nonident P-40Holey carbon on top of 400 mesh copper gridETHANE10090FEI VITROBOT MARK IBlot for 3.5 seconds before plunging.FEI POLARA 300FLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.3-1.8-2.831000.0OTHER2014-01-14GATAN K2 (4k x 4k)44Gatan K2 Summit in super-resolution counting mode. Motion correction as described in Li et al. (2013) Nature Methods.3D classification, refinement, and reconstruction were performed using RELION.Each particle7.6OTHERRELION128301NSFAFLEXIBLE FITChimera, PHENIXD2 domain of NSF was from crystal structure 1NSF. D1 domain of NSF was from related entry EMD-6204.R-factorRECIPROCAL