RELRCSBRCSBRCSB2015-11-232016-02-102016-02-102016-04-27Structure of the eukaryotic replicative CMG helicase and pumpjack motionYuan ZBai LSun JGeorgescu RELiu JO'Donnell MELi HCMG helicase, Cryo-EMYuan ZBai LSun JGeorgescu RELiu JO'Donnell MELi HStructure of the eukaryotic replicative CMG helicase and pumpjack motion.Nat.Struct.Mol.Biol.23217224201626854665doi:10.1038/nsmb.31703jc5FULLOVERLAPSaccharomyces cerevisiae CMG complexSaccharomyces cerevisiae CMG complex10.7CMG complexSaccharomyces cerevisiaeYeast0.71trueSaccharomyces cerevisiaesingleParticleparticle0.620 mM Tris acetate, 40 mM potassium glutamate, 2 mM DTT, 0.1 mM EDTA400 mesh holey carbon C-flat grid, glow-discharged in airETHANE90FEI VITROBOT MARK IVBlot for 3 seconds before plungingFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3001.53.529000.049505.0FEI TITAN KRIOS AUTOGRID HOLDER2015-08-01GATAN K2 SUMMIT (4k x 4k)800050All steps, including automatic particle picking, 2D classification, 3D classification, and 3D refinement were performed in Relion 1.4.CTFFIND44.7OTHERRelion_1.41785302Q9QRIGID BODY FITChimeraREAL