2018-04-11
REL
PDBj
PDBj
PDBj
2017-10-18
2018-04-11
2018-04-11
2018-04-11
Nucleosome with ALB1 enhancer DNA sequence
Takizawa Y
Tanaka H
Machida S
Koyama M
Maehara K
Ohkawa Y
Wade PA
Wolf M
Kurumizaka H
Takizawa Y
Tanaka H
Machida S
Koyama M
Maehara K
Ohkawa Y
Wade PA
Wolf M
Kurumizaka H
Cryo-EM structure of the nucleosome containing theALB1enhancer DNA sequence.
Open Biol
UK
8
2018
29563192
doi:10.1098/rsob.170255
2046-2441
EMD-6838
associated EM volume
Nucleosome with ALB1-DNA sequence
Nucleosome with ALB1-DNA sequence
Nucleosome with ALB1-DNA sequence
0
Homo sapiens
Escherichia coli
pET15b-H3.1, pET15b-H4, pET15b-H2A, pET15b-H2B, pGEM-T-Easy-(186 base-pair mouse ALB1 enhancer DNA)
.2
singleParticle
particle
.8
7.5
20.0
(HOCH2)3CNH2
Tris-HCl
1.0
Dithiothreitol
DTT
Quantifoil R1.2/1.3
COPPER
200
CARBON
HOLEY
100.0
PLASMA CLEANING
OTHER
9.33
Gatan Solarus
ETHANE
100
289
FEI VITROBOT MARK IV
3 second blot, 2.5uL.
sample was monodisperse
FEI TALOS ARCTICA
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
200
50.0
2.7
-1.5
-1.5
-3.0
-3.0
73000.
100000.
FEI TITAN KRIOS AUTOGRID HOLDER
NITROGEN
77.0
100.0
0.1
nanoprobe, parallel beam illumination
FEI FALCON III (4k x 4k)
INTEGRATING
4096
4096
14.0
1-40
1
2312
2.0
80.0
1
frame alignment and integration with motioncor2 incl. dose weighting
1182985
CTFFIND
4.1
deconvolution in RELION
PDB 3LZ0 low-pass filtered to 60A.
4
C2
FOURIER SPACE
4.0
FSC 0.143 CUT-OFF
RELION
2.1
288789
PROJECTION MATCHING
RELION
2.1
PROJECTION MATCHING
RELION
2.1
12
52212.
RELION
2.1