2019-12-25RELRCSBRCSBRCSB2018-01-232018-02-282019-01-302019-12-25National Science Foundation (NSF, United States)DMR-1344290United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM104427United StatesNational Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)R01HL115153United StatesDepartment of Energy (DOE, United States)DE-AC02-05CH11231United StatesSingle Molecule 3D Image of a Hole-Hole Homodimer Species Generated in the Production of a Bispecific Recombinant IgG1 (No. 017)Lei DLiu JLiu HLei MRen GLei DLiu JLiu HMarino JPLei MRen GSingle-Molecule 3D Images of "Hole-Hole" IgG1 Homodimers by Individual-Particle Electron Tomography.Sci RepUK988648864201931221961doi:10.1038/s41598-019-44978-72045-2322EMD-7369associated EM volumeIgG1 hole-hole homodimerIgG1 hole-hole homodimer0unidentifiedCricetulus griseusovarytomographyparticle7Dulbecco's phosphate buffered saline (DPBS)NEGATIVEUranyl FormateThe grid was stained with 1% (w/v) uranyl formateCOPPER200CARBONCONTINUOUSGLOW DISCHARGEAIR0.039NO SECTIONINGZEISS LIBRA120PLUSFLOOD BEAMBRIGHT FIELDLAB61202.280000.0In-column Omega FilterGATAN ULTRASCAN 4000 (4k x 4k)4096409670.751X-ray speckles in images were removed before CTF correction.FOURIER SPACE13.71FSC 0.5 CUT-OFFThe 3D reconstruction was performed by using Individual-Particle Electron Tomography (IPET). The obtained 3D map was Gaussian low-pass filtered to 2 nm. Image of the 3D map was taken in Chimera with the 'hide dust' function activated.61