2020-11-25RELRCSBRCSBRCSB2018-02-022018-03-142018-07-252020-11-25National Institutes of Health/National Institute Of Allergy and Infectious DiseasesR01 AI 120691United StatesStructure of the cargo bound AP-1:Arf1:tetherin-Nef closed trimer monomeric subunit after focussed classificationMorris KLBuffalo CZHurley JHMorris KLBuffalo CZSturzel CMHeusinger EKirchhoff FRen XHurley JHHIV-1 Nefs Are Cargo-Sensitive AP-1 Trimerization Switches in Tetherin Downregulation.Cell174659671.e14201830053425doi:10.1016/j.cell.2018.07.0041097-4172EMD-7456associated EM volumeEMD-7455other EM volumeEMD-7457other EM volumeEMD-7458other EM volumeClosed trimer assembly of AP-1:Arf1:Tetherin-NefClosed trimer assembly of AP-1:Arf1:Tetherin-Nef0Homo sapiensEscherichia coli0.23singleParticleparticle0.7820 mM Tris at pH 8.0, 200 mM NaCl, 5 mM MgCl2, and 0.5 mM TCEPC-flat-1.2/1.3COPPER300PLASMA CLEANINGETHANE100294FEI VITROBOT MARK IVAP-1:Arf1:Tetherin-NefFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30050.02.60.752.022500.046849.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION371238403-38122009.562.41252212RELIONRelion localized reconstruction (Ilca 2015)C14.05FSC 0.143 CUT-OFFRELION2.126684MAXIMUM LIKELIHOODRELIONMAXIMUM LIKELIHOODRELION2.1RELION2.1