2020-07-29RELRCSBRCSBRCSB2018-03-192018-04-112018-04-112020-07-29National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI127521United StatesNational Institutes of Health/Office of the DirectorS10OD021634United StatesNational Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)K99AI123498United StatesSARS Spike Glycoprotein, Trypsin-cleaved, Stabilized variant, one S1 CTD in an upwards conformationKirchdoerfer RNWang NPallesen JTurner HLCottrell CAMcLellan JSWard ABKirchdoerfer RNWang NPallesen JWrapp DTurner HLCottrell CACorbett KSGraham BSMcLellan JSWard ABStabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis.Sci RepUK81570115701201830356097doi:10.1038/s41598-018-34171-72045-2322EMD-7578associated EM volumeSARS Spike Glycoprotein, Trypsin-cleaved, Stabilized variant, one S1 CTD in an upwards conformation6cs0FULLOVERLAPSARS Spike Glycoprotein,Trypsin-cleaved, stabilized variantSARS Spike Glycoprotein,Trypsin-cleaved, stabilized variant01SARS coronavirusTor2Homo sapiensHEK293F0.54Spike glycoprotein,FibritinEnterobacteria phage T40.1347392663Homo sapiensLEVOSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATE
KSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVS
EKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYF
VGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATK
FPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLP
DDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQP
YRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDI
SPCAFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIG
AGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECAN
LLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADA
GFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGV
TQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEV
QIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQ
ERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEE
LDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQGSGYIPEAPRDGQAYVRKDGEWV
LLSTFLGRSLEVLFQ2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120815NAGsingleParticleparticle0.357.525.0TrisCl150.0NaClSodium chloride0.01A8-35C-flat-2/2 4CCOPPERCARBONHOLEYPLASMA CLEANINGOTHERETHANE100298FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.02.71.22.229000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING10.2565.01Gctf1.06Low-pass filtered negative-stain modelC13.8FSC 0.143 CUT-OFFRELION2.1139746ANGULAR RECONSTITUTIONRELION2.1ANGULAR RECONSTITUTIONRELION2.15I08A299-3235I08A610-11475X4SA18-2582AJFE323-502emd_7578_msk_1.mapemd_7578_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
320
320320
0
00320320320329.59998329.59998329.5999890.090.090.0XYZ-0.0197480060.04981966-0.000053873780.0024681381.031.031.03em-half-volume_P1emd_7578_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)