2020-01-22RELRCSBRCSBRCSB2018-03-272018-05-092018-09-192020-01-22National Institutes of Health/National Institute of Mental Health (NIH/NIMH)R01MH077303United StatesDepartment of Energy (DOE, United States)DE-AC02-05CH11231United StatesNational Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)R01HL115153United StatesSingle-Molecule 3D Image of neurexin 1 alpha by Individual Particle Electron Tomography (No. 029)Liu JFMisra AReddy SWhite MARen GRudenko GLiu JMisra AReddy MVVVSWhite MARen GRudenko GStructural Plasticity of Neurexin 1 alpha : Implications for its Role as Synaptic Organizer.J. Mol. Biol.UK43043254343201830193986doi:10.1016/j.jmb.2018.08.0261089-86380070JMOBAKEMD-7686associated EM volumeneurexin 1 alphaneurexin 1 alpha0Bos taurus0.137unidentified baculovirustomographyparticle825 mM Tris pH 8, 100 mM NaCl, 3 mM CaCl2NEGATIVEuranyl formateThe grid was stained for 15 s by sequential submersion in two drops of uranyl formate (UF).COPPER200CARBONCONTINUOUSGLOW DISCHARGEAIR0.039The purified neurexin 1 alpha proteins were stored in 25 mM Tris pH 8, 100 mM NaCl in flash-frozen aliquots.NO SECTIONINGZEISS LIBRA120PLUSFLOOD BEAMBRIGHT FIELDLAB61202.280000.0In-column Omega FilterGATAN ULTRASCAN 4000 (4k x 4k)40964096811.015.01X-ray speckles in images were removed before alignment and 3D reconstruction.FOURIER SPACE15.13FSC 0.5 CUT-OFFThe 3D reconstruction was performed by Individual-Particle Electron Tomography (IPET).65TOMOCTFIMODMicrographs were aligned together by IMOD. The CTF was corrected by TOMOCTF.