2020-05-27RELRCSBRCSBRCSB2018-05-152018-07-252019-05-152020-05-27National Institutes of Health/National Institute of General Medical SciencesR01 GM102474United StatesNational Institutes of Health/National Institute of General Medical SciencesU24GM116787United StatesNoodle-like structures in infectious microvesicles from poliovirus-infected HeLa cellsYang JEYang JERossignol EDChang DZaia JForrester IRaja KWinbigler HNicastro DJackson WTBullitt EComplexity and ultrastructure of infectious extracellular vesicles from cells infected by non-enveloped virus.Sci RepUK1079397939202032409751doi:10.1038/s41598-020-64531-12045-2322EMD-7877associated EM volumeEMD-7878other EM volumeEMD-7879other EM volumeEMD-7880other EM volumeEMD-7881other EM volumeHuman poliovirus 1 MahoneyHuman poliovirus 1 Mahoney0virion-containing microvesicles collected from supernatant of poliovirus-infected cells at 8 hours post-infectionHuman poliovirus 1 MahoneyHomo sapiensVIRUS-LIKE PARTICLESTRAINfalsefalsetomographyparticle1.571% glutaraldehyde in 1x PBSCOPPER200GLOW DISCHARGEAIRThe grid was coated with an extra layer of carbon (holes not covered) to provide extra support.ETHANE100283.15FEI VITROBOT MARK IIThe microvesicles from poliovirus-infected cells were collected through a series of centrifugations to enrich 100-1000 nm diameter membrane particles, followed by an annexin-V column purification to enrich phosphatidylserine-containing microvesicles.TED PELLA INC10NO SECTIONINGFEI TECNAI F20FLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN160TVIPS TEMCAM-F416 (4k x 4k)4.81BACK PROJECTIONeTomo47