2018-09-05RELRCSBRCSBRCSB2018-05-232018-06-202018-09-052018-09-05Negative Stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from rabbit 3420 at post boost 2.Ward ABTurner HLNogal BBianchi MTurner HLNogal BCottrell CAOyen DPauthner MBastidas RNedellec RMcCoy LEWilson IABurton DRWard ABHangartner LElectron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization.ImmunityUS49288300.e8201830097292doi:10.1016/j.immuni.2018.07.0091097-41802048IUNIEHEMD-7894associated EM volumeNegative stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from rabbit 3420 at post boost 2. Serum digested and Fab purified before adding trimer.Negative stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from rabbit 3420 at post boost 2. Serum digested and Fab purified before adding trimer.01234Oryctolagus cuniculussingleParticleparticle0.17.4TBSNEGATIVEUranyl formateHomemadeCOPPER400CELLULOSE ACETATEHOLEYGLOW DISCHARGEFEI TECNAI SPIRITFLOOD BEAMBRIGHT FIELDLAB6120SIDE ENTRY, EUCENTRICTVIPS TEMCAM-F416 (4k x 4k)40964096133825.01C120.0FSC 0.5 CUT-OFFRELION2.16001ANGULAR RECONSTITUTIONRELION2.1ANGULAR RECONSTITUTIONRELION2.1RELION2.1