2021-02-03RELRCSBRCSBRCSB2018-05-232018-06-202018-09-052021-02-03National Institutes of Health/National Institute Of Allergy and Infectious DiseasesAI100663United StatesBill & Melinda Gates FoundationOPP1115782United StatesNegative stain EM map of BG505 SOSIP.644 in complex with PGT121 and serum from rabbit 3417 PB1Turner HLWard ABNogal BBianchi MTurner HLNogal BCottrell CAOyen DPauthner MBastidas RNedellec RMcCoy LEWilson IABurton DRWard ABHangartner LElectron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization.ImmunityUS49288300.e8201830097292doi:10.1016/j.immuni.2018.07.0091074-76132048IUNIEHEMD-7904associated EM volumeComplex of BG505 SOSIP.664 with PGT121 and serum from rabbit 3417 PB1Complex of BG505 SOSIP.664 with PGT121 and serum from rabbit 3417 PB10Serum was purified through SEC after adding excess PGT121 and serumHuman immunodeficiency virusHomo sapiensHEK293FsingleParticleparticle0.1237.4TBSNEGATIVEUranyl Formateat 2%HomemadeCOPPER400CELLULOSE ACETATEGLOW DISCHARGESample in TBS, stained with UFFEI TECNAI SPIRITFLOOD BEAMBRIGHT FIELDLAB6120SIDE ENTRY, EUCENTRICTVIPS TEMCAM-F416 (4k x 4k)COUNTING1-4611800.525.01DogPicker was used to pick particles from micrographsCTFFIND35I8H1C1FOURIER SPACE18.45FSC 0.5 CUT-OFFRELION2.14563ANGULAR RECONSTITUTIONRELION2.1ANGULAR RECONSTITUTIONRELION2.1