2023-08-30RELRCSBRCSBRCSB2018-05-232018-06-062019-01-092023-08-30Two retinoschisin molecules interacting laterally forming a unit cell of a filamentous strand.Heymann JBVijayasarathy CHuang RKDearborn ADSieving PASteven ACRetinal adhesion protein X-linked retinoschisis Discoidin domain, CELL ADHESIONHeymann JBVijayasarathy CHuang RKDearborn ADSieving PASteven ACCryo-EM of retinoschisin branched networks suggests an intercellular adhesive scaffold in the retina.J. Cell Biol.US21810271038201930630865doi:10.1083/jcb.2018061481540-81402019JCLBA3EMD-6425other EM volumeSingle retinoschisin moleculeRetinoschisinRetinoschisin01Human retinoschisin with a honeybee mellitin leader sequence and a C-terminal 6-histidine tagHomo sapiensEyeRetinaIntercellular space0.38RetinoschisinHomo sapiensMature human RS1 with a C-terminal hexahistidine tagunidentified baculovirusLEVOSTEDEGEDPWYQKACKCDCQGGPNALWSAGATSLDCIPECPYHKPLGFESGEVTPDQITCSNPEQYVGWYSSWTANKARLNSQGFGCAWLSKFQDSSQWLQIDLKEIKVISGILTQGRCDIDEWMTKYSVQYRTDERLNWIYYKDQTGNNRVFYGNS
DRTSTVQNLLRPPIISRFIRLIPLGWHVRIAIRMELLECVSKCAHHHHHHO15537singleParticleparticle0.17.420.0NaHPO4Sodium phosphate500.0NaClSodium chloride200.0C3H4N2ImidazoleC-flat-2/2COPPER400CARBONHOLEY ARRAYPLASMA CLEANINGETHANE90293LEICA EM GPFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3001.01.043.03.23FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION1-50111360.250.6630 degree tilted specimen126702EMD-6425Limited to 20 angstromC2FOURIER SPACE10.0FSC 0.143 CUT-OFFBsoft1.9Reconstructed to 3 angstrom, low-pass filtered to 10 angstrom. Symmetrized two-fold (z-axis) and along screw-axis (x-axis).18646PROJECTION MATCHINGBsoft1.9PROJECTION MATCHINGBsoft1.9emd_7907_msk_1.mapemd_7907_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
224
224224
-112
-112-112224224224232.95999232.95999232.9599990.090.090.0XYZ-8.5165818.913717999999999-0.0089948431.36691491.041.041.04First half map of the RS1 unit cellemd_7907_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
224
224224
-112
-112-112224224224232.95999232.95999232.9599990.090.090.0XYZ-8.5517059.51605-0.0093701041.37278751.041.041.04Second half map of RS1 unit