2020-08-05RELRCSBRCSBRCSB2018-05-292019-01-162019-01-162020-08-05National Institutes of Health/National Institute of General Medical SciencesP41GM103832United StatesWelch FoundationQ1242United StatesFako virus empty particles, D2-symmetrized reconstructionKaelber JTJiang WWeaver SCAuguste AJChiu WKaelber JTJiang WWeaver SCAuguste AJChiu WArrangement of the Polymerase Complexes inside a Nine-Segmented dsRNA Virus.StructureUK28604612.e3202032049031doi:10.1016/j.str.2020.01.0110969-21262005STRUE6EMD-7941other EM volumeThe fully asymmetric structure from this dataset using alternate reconstruction techniquesEMD-7944other EM volumeThe high-resolution icosahedral reconstruction of the virionEMD-7945other EM volumeThe icosahedral reconstruction of this datasetEMD-7948associated EM volumeEMD-7949other EM volumeEMD-7953other EM volumeEMD-7954other EM volumeFako virusFako virus012345Fako virusCSW77Culicinae432VIRIONSTRAINfalsetrueclamp proteinFako virusCSW77LEVOMTLTYWDKEKRMTLKQMIQQVAINEQENELTHYVFTTPLSMPTFGKPMLGYVPLNEVATSKFFSNVNDFD
RDNQLAMAHFPDTTITQAYNLTNSIKPGDTSLPDAEVAALKWFWKFFTSINLVRQPPMDNVMYWACQFLS
SGTSFLPLERDVEIVFSGFKGSHICMFSNLRQMNLSPILCPYYDLITNFKTTTEIRAYVDAHEELKSLLT
YLCLCTIVGLCDTFTETRNMDTGEYVWKVRDVVSRNHTPAQNVEKFCYTIQNAKYMIQLVHVLLFPLTDN
KYADLPNYVAVITQGAINQSRSHNVINTTDESNSNTTSDTAASTSGIVSGDTGTVASLYPDEFKYVQSmajor capsid proteinFako virusCSW77LEVOMRPIRMYKNNQERTNLKHQEINEEQQNEQTTSNQGFTRSDNSGKINIERISSSRNQITDGKTVSSYSKIE
TNRSSQDSVQHGGSSITYTSDTTGNPRITNARTNNDETHATGPIEDLNSTSHGREPEIESFADRAELAMM
IQGMTVGALTVQPMRSIRSTFANLANVLIFHDVFTTEDKPSAFIEYHSDEMIVNMPKQTYNPIDNLAKIL
YLPSLEKFKYGTGIVQLNYSPHISKLYQNTNNIINTITDGITYANRTEFFIRVMVLMMMDRKILTMEFYD
VDTSAISNTAILPTIPTTTGVSPLLRIDTRTEPIWYNDAIKTLITNLTIQYGKIKTVLDANAVKRYSVVG
YPIDQYRAYLYNHNLLEYLGKKVKREDIMSLIKALSYEFDLITISDLEYQNIPKWFSDNDLSRFIFSICM
FPDIVRQFHALNIDYFSQANVFTVKSENAIVKMLNSNQNMEPTIINWFLFRICAIDKTVIDDYFSLEMTP
IIMRPKLYDFDMKRGEPVSLLYILELILFSIMFPNVTQHMLGQIQARILYISMYAFRQEYLKFITKFGFY
YKIVNGRKEYIQVTNQNERMTENNDVLTGNLYPSLFTDDPTLSAIAPTLAKIARLMKPTTSLTPDDRAIA
AKFPRFKDSAHLNPYSSLNIGGRTQHSVTYTRMYDAIEEMFNLILRAFASSFAQRPRAGVTQLKSLLTQL
ADPLCLALDGHVYHLYNVMANMMQNFIPNTDGQFHSFRACSYAVKDGGNIYRVVQNGDELNESLLIDTAI
VWGLLGNTDSSYGNAIGATGTANVPTKVQPVIPTPDNFITPTIHLKTSIDAICSVEGILLLILSRQTTIP
GYEDELNKLRTGISQPKVTERQYRRARESIKNMLGSGDYNVAPLHFLLHTEHRSTKLSKPLIRRVLDNVV
QPYVANLDPAEFENTPQLIENSNMTRLQIALKMLTGDMDDIVKGLILHKRACAKFDVYETLTIPTDVKTI
VLTMQHISTQTQNNMVYYVFLIDGVKILAEDIKNVNFQIDITGIWPEYVITLLLRAINNGFNTYVSMPNI
LYKPTITADVRQFMNTTKAETLLISNKSIVHEIMFFDNALQPKMSSDTLALSEAVYRTIWNSSIITQRIS
ARGLMNLEDARPPEAKISHQSELDMGKIDETSGEPIYTSGLQKMQSSKVSMANVVLSAGSDVIRQAAIKY
NVVRTQEIILFEturret proteinFako virusCSW77LEVOMIDLRLEEDILTATLPEFLSTRPKYRYAYTNTKQQDIRFQGPMRHVRLTHLYKQTKLWNLQYIERELAIS
EIDDALDEFIQTFSLPYVIEQGTYKYNMLLGMHAHNVNYQDDVSELIANNPQLLNYLDDNPFSAIFELVN
VDLQIYQYGQNIFNNEAEHTILFLKDNTNYGVIQALQKHPFSATHINWHLHKHIFVFHSREQLLNKLLSA
GLEDSQLYQRQKTYSTKRGDRPTERMVTYIEDDHIRRIQAVFPLLLDNIFDVKLHKDSSMTWLKSYADMI
YDSVKNSNSTITPEIRKLYLRMYNQYMRIFLPIEQYMLYDNTCWPFSEKITLKINVRLISSRENQPVLWK
TPIDTENLISIVQPDEPINKLNFTAIPSTMIRLNDNITMYRAVKDMFSAIEYLPDAIENIPTLTMKEQAL
SRYISPDSEAQNFFNNQPPYLNSIMNVNRQVFEAVKRGNIQVSTGSMEHLCLCMHVKSGLIVGRTVLIDD
KVVLRRNFNASTAKMITCYVKAFAQLYGEGSLINPGLRMVFFGVETEPAIDILKLFYGDKSLYIQGFGDR
GIGRDKFRTKIEDALTLRIGCDILISDIDQADYEDPNEEKFDDITDFVCYVTELVISNATVGLVKISMPT
YYIMNKISSTLNNKFSNVAINIVKLSTQKPYTYEAYIMLSHGSTLTNKGYLRNPVCDVYLEKISLQPMDL
KIISTISNEINYDKPTLYRFVVDKNDVTDVSIAMHILSIHCSTITTRSVMVRSDNTGAFVTMSGIKDMKR
VAIMNRMTDGTSANSYMHEQNGKLYLQKVPYLEDLISAFPNGFGSTYQNDYDSSMSVINVNALIRQVVYR
VISKSIPVALLESLSRIRIIGGRDLGEMNAVYKLYKTPIEVYDAVGITREYPHVQISYRAQRYSFTESIP
NHTLLLANYVIMNDVDGAPISSLEQINTIKKIISKISLGSIAYIQVYTDIVARNINVMTKNDSFLISANA
DKTVFKVQVSGYKAVEMCNYEQLLQLVSDNTGVNIIKLTYQDVLESCVLSSGILGDTGSWLLDLVLASTY
IIEIRGRNA-dependent RNA polymeraseFako virusCSW77LEVOMATEPNEINLRMTQTLMKQIEDTTFKIRRKHDDMYTYLIDDTAAIEMYPDTETPFRMKSSLHELYIQVKC
QIPKSIIRPPLYNNDMYPLNNNNTNFLEDRPFNYLCNFDWYEFLSRTKDELGMHYNMIRDLIAMSSQTRY
SNIWSNISGIIMYLRQYQRGFALRAILARLLKQWNNFPFFDTWDGFRDVLPNTSTAWPLLFYAFMSVTFD
YICENISEGEAIVCFQNHLENAQVSYQDTKLEKKNNYTLWLKYECHNLRVALTPRYDYTGTVSGNFLKDT
TEYIIENEFENNEIAMRNNLLPSFYAILQQMRQKLKTVEDIIRLLTICYSARDDRTYYGTLMELAISKAI
KPQVAGSIVPAPIPTSWLQKDPKMVLSARYPSTSFLSQMQEFYRRYYPALQKEIDVHALSASFINFLSTA
SAGVGIELPEEIVNMVQDKRLLYLIKKGSGKRVLQEALLVEKYNDLDPIINDFVRLIKIVVRKQIERRQR
GIAGIPNNVLKINQVTYEANKPFSKIARAPSHGKQSGNASDIHDLLFYTTQEDVSHIETNGKRQMRGIVI
SSADVKGMDTHIQINAAMNQHLGAIEILDGIQYDVGPFRQTNAIIQDIQGNVYEKSLNGGQQAIAFGLAN
FSQTTGINSKYFGQIPNQEGTFPSGLITTSNHHTQMLTLLIETALTTFTKEFGKSMAISHLMILGDDVSL
MLHGNDKDINFFMKYLVEKFSQLGLILERDESRNFGVFLQQHVINGRFNGFSNRIAIFTSEDYKTRKSVR
ESCTEYNALIDDVIFRTYNVRKLLQFQRIHQFVVLSKYVFRIQNYKYESLRAKLAKRMNVFEYELKIKDN
DKTDVHNRNALRFIGIQIPYTYFQYSGGGEIPPESFQKKDGSFTYEYSIYSPRGKWLRKFLYDISHDARD
VKFQIDHEMMKLYNLDICDFLLQYNVLDIQEEIRSTIIDRELVSKLAMNLESLEHSNARMISRRASESLR
SMGIRLPANGVYGYQINERLVKVLQNIQQSDYEVKMVGDTLFTAIMEKFEHHKVRMEKGDKLHNFTLDFS
NKDDKITINRKMLALHNISISKNMMPYSDAWMLYTCLDNTYNTSSDLATALAHSQGHFKSFQYDRDMFAE
SVKIASKHGIGSLPMELFFEASNIRDTAQLKWIEAIKYYVQFKDYLYPYSLNPRRLFFIPEQVSSVSNIL
NQENIPADINQKALLFRRAYAYVLSHPFCISGAKAIFVEDRISpolymerase accessory proteinFako virusCSW77LEVOMFAIPFTASQVYSHLGLYQQPIEKLDPLTKVQYESAEKLRDIITQLSRRSGGISLRKNLIRDIAFGNKPK
LNIPSLTHQVRHNREFKHGRGQFILVDSNGSDYYDGIFDDMVLILRSNFVLPNVPGIISEQLRLIVIDND
FYLKHIPSEIAILRDVLSEHMVKVSIDGVSLDDPYISKFFRKPFILYDHTKFRKVDLTKVVLINKTGKSK
AQLIDKYKLTDDAVFISYEVFDIMLQPVKSRDGDIEQFVRMRGDIETWKMKIGTTFESNLMELFIHGVPV
MNSTSQLQLDFQISDIRSANVFDEQIVCAAKLNGLREQCLKESSLIRVNIIGQKGSGKSMLMRLINERGI
PGISRKIICIDSDAYGKWKTQTQYLDDKFSALKLINVDNLHEISQDDNIISYYEQFIIDQLLAHNITISE
HTNSIRFIKQFKVDTLKDIGRKFKELYLADFKEQIHFYEYLCGNIPEPSSTLLITFLHATVETSAAPGTN
MNFSLNTILYPLQSILNRKRGALVNFILNRIYDEMGTEAFTRLRPCDVWKsingleParticleparticle7.810.0C4H12NO3ClTris-HCl1.0EDTA100.0NaClsodium chloridePLASMA CLEANINGunspecifiedETHANEFEI VITROBOT MARK IVJEOL 3200FSCFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3004.7JEOL 3200FSC CRYOHOLDERNITROGENIn-column Omega FilterDIRECT ELECTRON DE-20 (5k x 3k)INTEGRATING240030.019772jsprEMD-6001D2FOURIER SPACE5.6FSC 0.143 CUT-OFFjspr4886OTHERjsprPROJECTION MATCHINGjspremd_7948_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
576
576576
-288
-288-288576576576867.45605867.45605867.4560590.090.090.0XYZ-5.78868348.437480.0391874460.795378741.5061.5061.506D2-symmetrized map of Fako virus empty particles. Half set, unmaskedemd_7948_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)