2019-11-20RELRCSBRCSBRCSB2016-10-262016-12-282017-01-042019-11-20Howard Hughes Medical Institute (HHMI)United StatesJane Coffin Childs Memorial Fund61-1513United StatesCryo-electron microscopy structure of a bovine CLC-K chloride channel, main (class 1) conformationPark EMacKinnon RPark ECampbell EBMacKinnon RStructure of a CLC chloride ion channel by cryo-electron microscopy.NatureUK541500505201728002411doi:10.1038/nature208121476-46870006NATUASEMD-8435associated EM volumeCryo-electron microscopy structure of a bovine CLC-K chloride channel, main (class 1) conformationEMD-8454other EM volume5tqqunknownA bovine CLC-K channel complexed with a monoclonal antibody fragment (Fab)A bovine CLC-K channel complexed with a monoclonal antibody fragment (Fab)0123Fab fragment generated by proteolytic (papain) cleavage of murine IgG antibody.Bos taurusHomo sapienspFastBac (BacMam)Chloride channel proteinBos taurus0.0735214842Homo sapiensLEVOMRVRRGIRGGLDWLKRKLFCVGEDWYFLTVLGVLMALISFTMSFTVGRVVRAHKWLYREIGDSHLLRYLSWTVYPVALVS
FSSGFSQSITPFSGGSGIPELKTILSGVVLEDYLDIKNFGAKAVGLTCTLASGSTIFLGKVGPFVHLSVMIAAYLGRVRA
KATGESENKSKRNEMLVAGAAVGVATVFAAPFSGVLFCIEVVSSHFSVWDYWRGFFAATCGAFMFRLLAVFNSEQETITS
LYKTSFRVEVPFDLPEIFFFVALGAICGVASCAYLFCQRKFLGFVKTNPVLSKLMATSKPLYSALAALVLASVTYPPGAG
RFMASRLSMREYLDSLLDHNSWALLTRQASPPWPVEPDPQNLWFEWYHPQFTIFGTLAFFLVMKFWMLILATTIPMPAGY
FMPIFIFGAAIGRLLGEALSVAFPEGIVAGGVTNPIMPGGYALAGAAAFSGAVTHSISTALLAFELTGQIVHALPVLMAV
LAANAIAQSCQPSFYDGTIIVKKLPYLPWIRGRKISSHRVTVEHFMNRAITTLAKDTPQEEVVKVVTSTDMAEYPLVAST
ESQTLVGTMRRAQLVQALQAEPPSWAPGQQRCLQDILAEGCPVEPVTLKLSPETSLHQAHNLFELLNLQSLFVTSQGRAV
GFVSWVELEKAISKLTNPPAPKSNSLEVLFQMonoclonal antibody, Fab fragment, light chainMus musculus0.0118241022Mus musculusLEVODIVMTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKTLIYWASYRYSGVPDRFTGSGSGTDFTLAISNVQS
EDLAEYFCQQYNSYPLTFGSGTKLELKMonoclonal antibody, Fab fragment, heavy chainMus musculus0.0126330682Mus musculusLEVODVQLQESGPGLVKPSQSLSLTCTVTGDSVTSDYAWSWIRQFPGKKLEWMGYITYSGNTIYNPSLKSRISITRDTSKNQFF
LQLKSVIIEDTATYYCSRGVDYWGQGTSVTVSSsingleParticleparticle3.07.5100.0NaClsodium chloride20.0C4H11NO3Tris1.0C4H10O2S2DL-dithiothreitol0.04C24H46O11n-dodecyl-beta-D-maltoside0.004C31H50O4cholesteryl hemisuccinateQuantifoil R1.2/1.3COPPERCARBONHOLEY ARRAYETHANE90295FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION0.31.81CTFFIND4generated from EMAN2 and 2D averages of particle imagesC23.76FSC 0.143 CUT-OFFRELION1.4the indicated resolution was estimated on the primary map using the provided mask including Fab.82167RANDOM ASSIGNMENTRELION1.4NOT APPLICABLERELION1.4RELION1.4emd_8435_msk_1.mapemd_8435_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
240
240240
0
00240240240312.0312.0312.090.090.090.0XYZ-0.0711599740.1237781350.0001064677050.00415358271.31.31.3B-factor-sharpend (-100 A^2) and lowpass-filtered (3.7 Ang)