2020-01-15RELRCSBRCSBRCSB2017-02-242017-04-192017-04-192020-01-15Canadian Institutes of Health Research (CIHR)MOP-82930CanadaThe cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assemblyRazi AGuarne AOrtega JRazi AGuarne AOrtega JThe cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly.Proc. Natl. Acad. Sci. U.S.A.US114E3396E3403201728396444doi:10.1073/pnas.16180161141091-64900040PNASA6EMD-8626other EM volumeEMD-8621associated EM volumeThe cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assemblyEMD-8627other EM volumeEMD-8628other EM volume5uz4FULLOVERLAPStructure of the 30S subunit in complex with YjeQ GTPaseStructure of the 30S subunit in complex with YjeQ GTPase0123456789101112131415161718192021Escherichia coli16S RIBOSOMAL RNAEscherichia coli0.4949711881GAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAAGCUU
GCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGG
UAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGGGAUU
AGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAACUGA
GACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGU
GUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUGACGU
UACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAUUACU
GGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUA
GCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCGGUGG
CGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGUAGUC
CACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUG
GGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAU
GCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGUGAGAC
AGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCUUUGU
UGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCAUCAU
GGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGACCUCA
UAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUG
CCACGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUAGCUU
AACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACCUGCG
GUUGGAU30S ribosomal protein S3Escherichia coli0.0260313161LEVOMGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKK
GEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK30S ribosomal protein S4Escherichia coli0.0235141991LEVOMARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAA
RLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK30S ribosomal protein S5Escherichia coli0.0176293981LEVOMAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGT
LQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
VEEILGK30S ribosomal protein S6Escherichia coli0.0152110581LEVOMRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRF
NDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANETADDAEAGDSEE30S ribosomal protein S7Escherichia coli0.0200551561LEVOMPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRV
GGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLR
SFSHQAGASSKQPALGYLN30S ribosomal protein S8Escherichia coli0.0141465571LEVOMSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSR
PGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA30S ribosomal protein S9Escherichia coli0.014886271LEVOMAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIR
HGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRKARRRPQFSKR30S ribosomal protein S10Escherichia coli0.0117555971LEVOMQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPT
EKTVDALMRLDLAAGVDVQISLG30S ribosomal protein S11Escherichia coli0.0138709751LEVOMAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIK
NLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV30S ribosomal protein S12Escherichia coli0.0137681571LEVOMATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI
LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA30S ribosomal protein S13Escherichia coli0.0131284671LEVOMARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRL
MDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK30S ribosomal protein S14Escherichia coli0.011606561LEVOMAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNAVLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLS
RIKVREAAMRGEIPGLKKASW30S ribosomal protein S15Escherichia coli0.0103198821LEVOMSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTR
LIERLGLRR30S ribosomal protein S16Escherichia coli0.0092075721LEVOMVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNK
AA30S ribosomal protein S17Escherichia coli0.0097244911LEVOMTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVE
KAVL30S ribosomal protein S18Escherichia coli0.0090054721LEVOMARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRHQ30S ribosomal protein S19Escherichia coli0.0104553551LEVOMPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNMIGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTY
RGHAADKKAKKK30S ribosomal protein S20Escherichia coli0.0097084641LEVOMANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLT
AQINKLA30S ribosomal protein S2Escherichia coli0.026781671LEVOMATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKIASRKGKILFVGTKRAASEAV
KDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEA
ESmall ribosomal subunit biogenesis GTPase RsgAEscherichia coli0.0373451721Escherichia coliLEVOLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIVISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGK
PAAEGVNVKGIVEAVHERTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLNKIDLL
DDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGVGKSSLLNALLGLQKEILTNDISDNSGL
GQHTTTAARLYHFPHGGDVIDSPGVREFGLWHLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETR
FENYHRILESMAQV3.6.1.-ZINC ION6.5409e-051ZN3'-O-(N-methylanthraniloyl)-beta:gamma-imidoguanosine-5'-triphosphate0.0006553431GGMsingleParticleparticle7.5C-flat CFT-222CCOPPER200CARBONHOLEYGLOW DISCHARGEETHANE100298FEI VITROBOT MARK IIIFEI TECNAI F20FLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN20050.02.00.53.025000.034482.0GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING1-200.51.014870182AVY5.8FSC 0.143 CUT-OFF130462PROJECTION MATCHINGRELION1.4PROJECTION MATCHINGRELION1.4RELION1.4OTHER