2019-11-20RELRCSBRCSBRCSB2017-07-102017-08-022017-08-022019-11-20National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)NS093838United StatesNational Institutes of Health/National Cancer Institute (NIH/NCI)CA206573United StatesNational Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)NS083660United StatesHoward Hughes Medical Institute (HHMI)United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM029169United StatesActivated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitoninTwomey ECYelshanskaya MVGrassucci RAFrank JSobolevsky AITwomey ECYelshanskaya MVGrassucci RAFrank JSobolevsky AIChannel opening and gating mechanism in AMPA-subtype glutamate receptors.NatureUK5496065201728737760doi:10.1038/nature234790028-08360006NATUASEMD-8823associated EM volumeActivated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitoninEMD-8819other EM volumeEMD-8820other EM volumeEMD-8821other EM volumeEMD-8822other EM volume5weoFULLOVERLAPActivated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitoninActivated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitonin01Rattus norvegicusHomo sapiensHEK293 gnti-Glutamate receptor 2,Voltage-dependent calcium channel gamma-2 subunit chimeraMus musculus0.1155019694Homo sapiensLEVONSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFC
GTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNIN
NDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGAEVSGFQIVD
YDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEI
ERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVLTEDDTSGLEQKTVVVTTILESPYVMMK
KNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVI
DFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTN
EFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGT
LDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDS
KGYGIATPKGSSLGTPVNLAVLKLSEQGVLDKLKNKWWYDKGECGAKDSGSKEKTSALSLSNVAGVFYILVGGLGLAMLV
ALIEFCYKSRAEAKRMKGTGLFDRGVQMLLTTVGAFAAFSLMTIAVGTDYWLYSRGVCKTKSVSEDETSKKNEEVMTHSG
LWRTCCLEGNFKGLCKQIDHFPEDADYEADTAEYFLRAVRASSIFPILSVILLFMGGLCIAASEFYKTRHNIILSAGIFF
VSAGLSNIIGIIVYISANAGDPSKSDSKKNSYSYGWSFYFGALSFIIAEMVGVLAVHMFIDRHKQLTGGLVPRGGLUTAMIC ACID0.0001471294GLUCYCLOTHIAZIDE0.0003898784CYZsingleParticleparticle48C-flat-1.2/1.3GOLD200ETHANE100295FEI VITROBOT MARK IVActivated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitoninFEI TITAN KRIOSSPOT SCANBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)COUNTING8.055.015KBUGluA2-2xSTZ in DDMC24.2FSC 0.143 CUT-OFFTMD map uploaded under additional files is at 4.0 angstrom resolution.69207ANGULAR RECONSTITUTIONANGULAR RECONSTITUTIONREALemd_8823_additional.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
300
300300
0
00300300300324.0324.0324.090.090.090.0XYZ-0.140595610.241352280.00059603040.0055290681.081.081.08Focused TMD refinement of activated complex of GluA2 bound to glutamate, cyclothiazide and STZ