2020-12-02RELRCSBRCSBRCSB2018-07-052018-10-312019-05-222020-12-02National Institutes of Health/National Cancer Institute (NIH/NCI)Intramural Research ProgramUnited StatesCryo-EM structure of nucleosome in complex with a single chain antibody fragmentYadav KNSZhou B-RZhou BRYadav KNSBorgnia MHong JCao BOlins ALOlins DEBai YZhang PAtomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment.Nat CommunUK1023012301201931127102doi:10.1038/s41467-019-10247-42041-1723EMD-8938associated EM volumeCryo-EM structure of nucleosome in complex with a single chain antibody fragmentEMD-8945other EM volumeEMD-8949other EM volume6dztFULLOVERLAPNucleosome-Antibody complexNucleosome-Antibody complex01234567Drosophila melanogasterEscherichia coliHistone H3Drosophila melanogaster0.0154211012Escherichia coliLEVOMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFK
TDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERAHistone H4Drosophila melanogaster0.0115216112Escherichia coliLEVOMITGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKR
KTVTAMDVVYALKRQGRTLYGFGGHistone H2ADrosophila melanogaster0.0133887272Escherichia coliLEVOMSGRGKGGKVKGKAKSRSNRAGLQFPVGRIHRLLRKGNYAERVGAGAPVYLAAVMEYLAAEVLELAGNAARDNKKTRIIP
RHLQLAIRNDEELNKLLSGVTIAQGGVLPNIQAVLLPKKTEKKAHistone H2BDrosophila melanogaster0.0138402242Escherichia coliLEVOMIPPKTSGKAAKKAGKAQKNITKTDKKKKRKRKESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLA
HYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKDNA (147-MER)Escherichia coli0.0456100431(DA)(DT)(DC)(DG)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)
(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)
(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)
(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)
(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)
(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)
(DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)
(DT)(DC)(DT)(DC)(DG)(DA)(DT)DNADNA (147-MER)Escherichia coli0.045138771(DA)(DT)(DC)(DG)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)
(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)
(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)
(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)
(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)
(DG)(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)
(DG)(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)
(DA)(DT)(DC)(DC)(DG)(DA)(DT)DNAscFvMus musculus0.0290301462Escherichia coliLEVOMKSSHHHHHHENLYFQSNAMEVQLQQSGPELVEPGTSVKMPCKASGYTFTSYTIQWVKQTPRQGLEWIGYIYPYNAGTKY
NEKFKGKATLTSDKSSSTVYMELSSLTSEDSAVYYCARKSSRLRSTLDYWGQGTSVTVSSGGGGSGGGGSGGGGSMDIKM
TQSPSSMHASLGERVTITCKASQDIRSYLSWYQQKPWKSPKTLIYYATSLADGVPSRFSGSGSGQDFSLTINNLESDDTA
TYYCLQHGESPYTFGSGTKLEIKRAsingleParticleparticle7.4ETHANEFEI TITAN KRIOSOTHEROTHERFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)40.013LZ0input pdb models: 3LZ0, 1KX5, 2GKI2C12.99FSC 0.143 CUT-OFFRELION3.0 beta238790NOT APPLICABLENOT APPLICABLEemd_8938_msk_1.mapemd_8938_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
220
220220
0
00220220220186.34186.34186.3490.090.090.0XYZ-0.0151343580.028581262-0.000013729320.00190772380.8470.8470.847emd_8938_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)