2019-11-27RELRCSBRCSBRCSB2018-07-082018-08-222018-08-222019-11-27National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R21 AI125983United StatesNational Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)T32 AI007323United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01 GM071940United StatesNational Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)R01 DE025567United StatesNational Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI094386United StatesNational Institutes of Health/National Center for Research Resources (NIH/NCRR)S10RR23057United StatesNational Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)K99/R00 HL133453United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)U24GM116792United StatesNational Institutes of Health/Office of the DirectorS10OD018111United StatesNational Science Foundation (NSF, United States)DMR-1548924United StatesNational Science Foundation (NSF, United States)DBI-1338135United StatesPTEX Core Complex in the Engaged (Extended) StateHo CLai MZhou ZHHo CMBeck JRLai MCui YGoldberg DEEgea PFZhou ZHMalaria parasite translocon structure and mechanism of effector export.NatureUK5617075201830150771doi:10.1038/s41586-018-0469-40028-08360006NATUASEMD-8951associated EM volumePTEX Core Complex in the Engaged (Extended) StateEMD-8952other EM volumePTEX Core Complex in the Resetting (Compact) State6e10FULLOVERLAPPlasmodium Translocon of Exported Proteins (PTEX) Core ComplexPlasmodium Translocon of Exported Proteins (PTEX) Core Complex012345Plasmodium falciparum 3D7Heat shock protein 101isolate 3D70.1062771256LEVOMTRRYLKYYIFVTLLFFVQVINNVLCAPDNKQEQGKYLNRTINILNAGKNIAKSYGHNKLKPIHILSALAKSDYGSTLFK
ENNVNAANLKEYIDIALEQTRAGAPLDNKSKIVNSAEVKETLALAEAAANKYKSPKVDVEHLLSGLSNDELVNEIFNEVY
LTDEAIKAILKRKFEKTKKDKDGKTGTLYIEQFGSNMNEKVRNGKLQGIYGRDEEIRAIIESLLRYNKNSPVLVGNPGTG
KTTIVEGLVYRIEKGDVPKELQGYTVISLNFRKFTSGTSYRGEFETRMKNIIKELKNKKNKIILFVDEIHLLLGAGKAEG
GTDAANLLKPVLSKGEIKLIGATTIAEYRKFIESCSAFERRFEKILVEPPSVDMTVKILRSLKSKYENFYGINITDKALV
AAAKISDRFIKDRYLPDKAIDLLNKACSFLQVQLSGKPRIIDVTERDIERLSYEISTLEKDVDKVSKKKYNKLIKEFEEK
KEQLKKYYEEYVITGERLKRKKEIEKKLNDLKELTQNYVYSNKEPPIELQNSLKEAQQKYLELYKETVAYVEAKTHNAMN
VDAVYQEHVSYIYLRDSGMPLGSLSFESSKGALKLYNSLSKSIIGNEDIIKSLSDAVVKAATGMKDPEKPIGTFLFLGPT
GVGKTELAKTLAIELFNSKDNLIRVNMSEFTEAHSVSKITGSPPGYVGFSDSGQLTEAVREKPHSVVLFDELEKAHADVF
KVLLQILGDGYINDNHRRNIDFSNTIIIMTSNLGAELFKKKLFFDADNSGTPEYKRVMEDVRLSLIKKCKKVFKPEFVNR
IDKIGVFEPLNKKNLHKIVALRFKKLEKRLEEKNIQVSVSEKAIDYIIDQSYDPELGARPTLIFIESVIMTKFAIMYLKK
ELVDDMDVFVDYNSKAKNLVINLSKTPRDYKDDDDKDYKDDDDKDYKDDDDKExported protein 2isolate 3D70.0334587077LEVOMKVSYIFSFFLLFFVYKNTNTVVCDNGYGDLAATSALTTVIKDPISLTIKDIYEHGVKNPFTKIIHKLKKFIRYRKVLRW
SRMWWVLLVREIVGDNTIEKKTEKALREIWDQCTIAVYNNTLNAVESKPLLFLHGILNECRNNFATKLRQDPSLIVAKID
QIIKSQIYRFWVSEPYLKIGRSHTLYTHITPDAVPQLPKECTLKHLSSYMEEKLKSMESKKNIESGKYEFDVDSSETDST
KDDGKPDDDDDDDDNFDDDDNFDDDTVEEEDASGDLFKNEKKDENKETranslocon component PTEX150isolate 3D70.022593178LEVOSVKDIKKLIEEGILDYEDLTENELRKLAKPDDNFYELSPYASDEKDLSLNETSGLTNEQLKNFLGQNGTYHMSYDSKSID
YAKQKKSEKKEDQQEDDDGFYDAYKQIKNSYDGIPNNFNHEAPQLIGNNYVFTSIYDTKENLIKFLKKNSEYDLYD
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(UNK)(UNK)(UNK)Endogenous cargo polypeptideisolate 3D70.0012945871LEVO(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)Unknown (Claw)isolate 3D70.0049540986LEVO(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
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(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER0.00052324712AGSsingleParticleparticle7.4COPPERCARBONLACEYGLOW DISCHARGEETHANEFEI VITROBOT MARK IVPTEX core complex purified from P. falciparum parasites cultured in human erythrocytesFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.02.72.01.54.04.0130000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGIF Quantum LS20GATAN K2 SUMMIT (4k x 4k)COUNTING371038382-5010.060.011508462CTFFINDCTFFIND4.14.16FSC 0.143 CUT-OFFRELION2.072866PROJECTION MATCHINGcryoSPARCPROJECTION MATCHINGRELIONRELION2.0AB INITIO MODELREALemd_8951_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
480
480480
0
00480480480499.19998499.19998499.1999890.090.090.0XYZ-0.062106110.145062330.000203994630.0028656751.041.041.04PTEX Core Complex in the Engaged (Extended) State, additional map #1emd_8951_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
480
480480
0
00480480480499.19998499.19998499.1999890.090.090.0XYZ-0.290539230.443076670.000113221980.0048744191.041.041.04PTEX Core Complex in the Engaged (Extended) State, additional map #2