2024-03-13RELRCSBRCSBRCSB2018-07-132018-08-082018-12-262024-03-13National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)5SC1AI114843United StatesMechanism of cellular recognition by PCV2Khayat RDhindwal Sviral jelly-roll, VIRUS LIKE PARTICLEDhindwal SAvila BFeng SKhayat RPorcine Circovirus 2 Uses a Multitude of Weak Binding Sites To Interact with Heparan Sulfate, and the Interactions Do Not Follow the Symmetry of the Capsid.J.Virol.US93201930602608doi:10.1128/JVI.02222-181098-55140825JOVIAMEMD-8939other EM volumeEMD-8969other EM volumeEMD-8970other EM volumeEMD-8971other EM volumeEMD-8972other EM volumeEMD-8973other EM volumeEMD-8974other EM volume6e39FULLOVERLAPPorcine circovirus 2Porcine circovirus 201Porcine circovirus 2Sus scrofa1.4Capsid protein215.01VIRUS-LIKE PARTICLESPECIESfalsefalsePCV2 capsid11A segment of heparin sulfate identified in the image reconstructionSus scrofaHeparin sulfate1Sus scrofaCapsid protein of PCV2Porcine circovirus 20.02789979499999999860Trichoplusia niLEVOMTYPRRRYRRRRHRPRSHLGQILRRRPWLVHPRHRYRWRRKNGIFNTRLSRTFGYTIKRTTVKTPSWAVDMMRFNINDFL
PPGGGSNPRSVPFEYYRIRKVKVEFWPCSPITQGDRGVGSSAVILDDNFVTKATALTYDPYVNYSSRHTITQPFSYHSRY
FTPKPVLDSTIDYFQPNNKRNQLWLRLQTAGNVDHVGLGTAFENSIYDQEYNIRVTMYVQFREFNLKDPPLNPQ805N7singleParticleparticle0.7187.020.0HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)250.0NaClSodium Chloride0.1TCEPTris(2-carboxyethyl)phosphine2.0EDTAEthylenediaminetetraacetic acidCARBONLACEYunspecifiedETHANE1004FEI VITROBOT MARK IVThis sample was monodisperseFEI TITANFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300100.00.01NITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING2-25137255.032.011663693R0Rlow pass filtered to 60 AngstromsI3.5FSC 0.143 CUT-OFFFREALIGN9.11No refinement of parameters66701MAXIMUM LIKELIHOODRELION2.0MAXIMUM LIKELIHOODRELION1FREALIGN9,11FLEXIBLE FITSeveral iterations of refinementCorrelation coefficientREAL35.5